Rb X Ms Connexin 43 (Cx43), C-terminus

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	ELISA, IF, IHC, IP, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

ELISA, IF, IHC, IP, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB1728
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Connexin 43 Antibody, CT, cytosolic
Overview	Mouse Connexin 43 is a 382 amino acid gap junction protein with a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).
Alternate Names	Gap Junction alpha-1 Protein (CxA-1)
Background Information	Connexin 43 (GJA1) is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5. Mouse Connexin 43 is a 382 amino acid gap junction protein with a predicted M.W. of ~43 kDa. It is prominently expressed in heart (Kumar, N. and Giula, N., 1996, Cell 84: 381-388).

Description

Catalogue Number

AB1728

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Connexin 43 Antibody, CT, cytosolic

Overview

Mouse Connexin 43 is a 382 amino acid gap junction protein with a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).

Alternate Names

Gap Junction alpha-1 Protein (CxA-1)

Background Information

Connexin 43 (GJA1) is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5. Mouse Connexin 43 is a 382 amino acid gap junction protein with a predicted M.W. of ~43 kDa. It is prominently expressed in heart (Kumar, N. and Giula, N., 1996, Cell 84: 381-388).

Product Information
Format	Affinity Purified
Control	Positive Control: Heart tissue, mouse brain tissue lysate.
Presentation	Purified rabbit polyclonal in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, with 0.1% sodium azide as a preservative.

Product Information

Format

Affinity Purified

Control

Positive Control: Heart tissue, mouse brain tissue lysate.

Presentation

Purified rabbit polyclonal in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, with 0.1% sodium azide as a preservative.

Applications
Application	Anti-Connexin 43 Antibody, C-terminus, cytosolic is an antibody against Connexin 43 for use in ELISA, IF, IH, IP & WB.
Key Applications	ELISA Immunofluorescence Immunohistochemistry Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: 2-10 µg of a previous lot was used in immunoprecipitation. ELISA: A previous lot of this antibody was used at 1:10,000-100,000 dilution using 50 - 100 ng Cx43 control peptide (Catalog number AG636) per well. Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Connexin 43 Antibody, C-terminus, cytosolic is an antibody against Connexin 43 for use in ELISA, IF, IH, IP & WB.

Key Applications

ELISA
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Western Blotting

Application Notes

Immunoprecipitation:
2-10 µg of a previous lot was used in immunoprecipitation.

ELISA:
A previous lot of this antibody was used at 1:10,000-100,000 dilution using 50 - 100 ng Cx43 control peptide (Catalog number AG636) per well.

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Anti-Connexin 43 is made against a 23 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse C×43 (Beyer, E. and Steinberg, T. ,1991, JBC 266: 7971).
Epitope	C-term/cytosolic
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes mouse Connexin 43.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Mouse C×43 immunogenic peptide sequence is specific for C×43 and no significant homology is seen with other connexins. The mouse C×43 peptide sequence is 100% homologous with rat and human C×43 (Beyer, E. et al., 1985, JCB 105: 2621).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_000156
Entrez Gene Summary	This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq]
Gene Symbol	GJA1 GJAL Cx43 DFNB38 SDTY3 CX43 ODD ODDD ODOD Connexin-43
Purification Method	ImmunoAffinity Purified
UniProt Number	P17302
UniProt Summary	FUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. SIZE: 382 amino acids; 43008 Da SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea. DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis. SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.
Molecular Weight	43 kDa

Biological Information

Immunogen

Anti-Connexin 43 is made against a 23 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse C×43 (Beyer, E. and Steinberg, T. ,1991, JBC 266: 7971).

Epitope

C-term/cytosolic

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes mouse Connexin 43.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Mouse C×43 immunogenic peptide sequence is specific for C×43 and no significant homology is seen with other connexins. The mouse C×43 peptide sequence is 100% homologous with rat and human C×43 (Beyer, E. et al., 1985, JCB 105: 2621).

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_000156

Entrez Gene Summary

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq]

Gene Symbol

GJA1
GJAL
Cx43
DFNB38
SDTY3
CX43
ODD
ODDD
ODOD
Connexin-43

Purification Method

ImmunoAffinity Purified

UniProt Number

P17302

UniProt Summary

FUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
SIZE: 382 amino acids; 43008 Da
SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea.
DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.

Molecular Weight

43 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on Huvec lysates. Western Blot Analysis: 1:500 dilution of this lot detected CONNEXIN 43 on 10 μg of Huvec lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected CONNEXIN 43 on 10 μg of Huvec lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for up to 1 year at 2-8°C in undiluted aliquots from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for up to 1 year at 2-8°C in undiluted aliquots from date of receipt.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Rb X Ms Connexin 43 (Cx43), C-terminus

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