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Rb X Ovine IL-8

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货号：AB1840

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Sh ELISA Rb Serum Polyclonal Antibody
Description
Catalogue Number AB1840
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Interleukin-8 Antibody
Alternate Names
  • IL-8
References
Product Information
Format Serum
Presentation Rabbit serum. Liquid containing 0.1% sodium azide.
Applications
Application Anti-Interleukin-8 Antibody detects level of Interleukin-8 & has been published & validated for use in ELISA.
Key Applications
  • ELISA
Application Notes Detecting antibody in ELISA, using monoclonal anti-ovine IL-8 as capture antibody. Recommended dilution factor 1:500. See suggested protocol.

Optimal working dilutions must be determined by the end user.

Suggested ELISA Protocol For Ovine IL-8

1. Coat high capacity microtiter wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.

2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).

3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.

4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.

5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.

6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.

Notes:

The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.

Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.

Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
Biological Information
Immunogen Recombinant ovine IL-8
Host Rabbit
Specificity Reacts with ovine IL-8. No reactivity detected to recombinant ovine IL-1beta, IL-6, MCP or TNFalpha.
Species Reactivity
  • Sheep
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
Gene Symbol
  • IL8
  • GCP-1
  • emoctakin
  • MDNCF
  • NAP1
  • CXCL8
  • AMCF-I
  • IL-8
  • Emoctakin
  • TSG-1
  • NAP-1
  • LYNAP
  • MONAP
  • SCYB8
  • GCP1
  • LUCT
  • LECT
  • K60
  • 3-10C
  • b-ENAP
  • NAF
UniProt Number
UniProt Summary FUNCTION: SwissProt: P10145 # IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
SIZE: 99 amino acids; 11098 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
PTM: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
SIMILARITY: SwissProt: P10145 ## Belongs to the intercrine alpha (chemokine CxC) family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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