Anti-MMP-9, clone EP1254, Rabbit Monoclonal

Species Reactivity	Key Applications	Host	Format	Antibody Type
Gp, H	WB, IHC, IH(P)	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Gp, H

WB, IHC, IH(P)

Purified

Polyclonal Antibody

Description
Catalogue Number	AB13458
Replaces	04-1150
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-MMP-9 Antibody
Alternate Names	92 kDa Type IV Collagenase 92 kDa gelatinase Gelatinase B GELB
Background Information	Matrix metalloproteinases (also named matrixin or MMPs) are a major group of enzymes that regulate cell-matrix composition by using zinc for their proteolytic activities. They are essential for various normal biological processes such as embryonic development, morphogenesis, reproduction tissue resorption, and remodeling. Metalloproteinases also play a role in pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and cancer. The metalloproteinase MMP-9 is associated with a variety of autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, systemic sclerosis, rheumatoid arthritis, multiple sclerosis, polymyositis and atherosclerosis. MMP-9 plays either a primary or secondary role in each one of these autoimmune diseases by its up or down-regulation. It is not expressed constantly but rather is induced or suppressed by many regulating molecules. This feature of MMP-9 along with its involvement in disease pathogenesis turns it into a target for therapy in the treatment of potentially many autoimmune diseases. Like other proteolytic enzymes, MMP-9 is first synthesized as inactive proenzyme or zymogens. activation of proMMP-9 is mediated by plasminogen activator/plasmin (PA/plasmin) system. The regulation of MMP-9 activity is also controlled through TIMP-3. MMP-9 expression is regulated by several cytokines and growth factors, including interleukins, interferons, EGF, NGF, bFGF, VEGF, PDGF, TNF-alpha, TGF-beta, the extracellular matrix metalloproteinase inducer EMMPRIN and also osteopontin. Many of these stimuli induce the expression and/or activation of c-fos and c-jun proto-oncogene products, which heterodimerize and bind activator protein-1 (AP-1) sites within of MMP9 gene promoters. Normally MMP-9’s primary function is the degradation of the extracellular matrix. It can degrade decorin, elastin, fibrillin, laminin, gelatin (denatured collagen), and types IV, V, XI and XVI collagen and also activates growth factors like proTGF-beta and proTNF-alpha. Physiologically, MMP-9 in coordination with other MMPs, play a role in normal tissue remodeling events such as neurite gowth, embryonic development, angiogenesis, ovulation, mammary gland involution and wound healing. MMP-9 with other MMPs is also involved in osteoblastic bone formation and/or inhibits osteoclastic bone resorption. {http://atlasgeneticsoncology.org/Genes/MMP9ID41408ch20q11.html}.

Description

Catalogue Number

AB13458

Replaces

04-1150

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-MMP-9 Antibody

Alternate Names

92 kDa Type IV Collagenase
92 kDa gelatinase
Gelatinase B
GELB

Background Information

Matrix metalloproteinases (also named matrixin or MMPs) are a major group of enzymes that regulate cell-matrix composition by using zinc for their proteolytic activities. They are essential for various normal biological processes such as embryonic development, morphogenesis, reproduction tissue resorption, and remodeling. Metalloproteinases also play a role in pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and cancer. The metalloproteinase MMP-9 is associated with a variety of autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, systemic sclerosis, rheumatoid arthritis, multiple sclerosis, polymyositis and atherosclerosis. MMP-9 plays either a primary or secondary role in each one of these autoimmune diseases by its up or down-regulation. It is not expressed constantly but rather is induced or suppressed by many regulating molecules. This feature of MMP-9 along with its involvement in disease pathogenesis turns it into a target for therapy in the treatment of potentially many autoimmune diseases.
Like other proteolytic enzymes, MMP-9 is first synthesized as inactive proenzyme or zymogens. activation of proMMP-9 is mediated by plasminogen activator/plasmin (PA/plasmin) system. The regulation of MMP-9 activity is also controlled through TIMP-3.
MMP-9 expression is regulated by several cytokines and growth factors, including interleukins, interferons, EGF, NGF, bFGF, VEGF, PDGF, TNF-alpha, TGF-beta, the extracellular matrix metalloproteinase inducer EMMPRIN and also osteopontin. Many of these stimuli induce the expression and/or activation of c-fos and c-jun proto-oncogene products, which heterodimerize and bind activator protein-1 (AP-1) sites within of MMP9 gene promoters.
Normally MMP-9’s primary function is the degradation of the extracellular matrix. It can degrade decorin, elastin, fibrillin, laminin, gelatin (denatured collagen), and types IV, V, XI and XVI collagen and also activates growth factors like proTGF-beta and proTNF-alpha. Physiologically, MMP-9 in coordination with other MMPs, play a role in normal tissue remodeling events such as neurite gowth, embryonic development, angiogenesis, ovulation, mammary gland involution and wound healing. MMP-9 with other MMPs is also involved in osteoblastic bone formation and/or inhibits osteoclastic bone resorption. {http://atlasgeneticsoncology.org/Genes/MMP9ID41408ch20q11.html}.

Product Information
Format	Purified
Control	POSITIVE CONTROL: Conditioned, serum-free medium from TPA-treated human fibrosarcoma (HT-1080) cells. Placenta or breast carcinoma.
Presentation	Purified immunogobulin by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.

Product Information

Format

Purified

Control

POSITIVE CONTROL:

Conditioned, serum-free medium from TPA-treated human fibrosarcoma (HT-1080) cells. Placenta or breast carcinoma.

Presentation

Purified immunogobulin by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.

Applications
Application	Anti-MMP-9 Antibody detects level of MMP-9 & has been published & validated for use in WB, IH, IH(P).
Key Applications	Western Blotting Immunohistochemistry Immunohistochemistry (Paraffin)
Application Notes	Immunohistology (formalin/paraffin): 10-20μg/ml for 30 min at RT. No special pretreatment is required for histochemical staining of formalin/paraffin tissues. Western Blot: 2-4μg/mL for 2 hours at RT Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-MMP-9 Antibody detects level of MMP-9 & has been published & validated for use in WB, IH, IH(P).

Key Applications

Western Blotting
Immunohistochemistry
Immunohistochemistry (Paraffin)

Application Notes

Immunohistology (formalin/paraffin): 10-20μg/ml for 30 min at RT. No special pretreatment is required for histochemical staining of formalin/paraffin tissues.

Western Blot: 2-4μg/mL for 2 hours at RT

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Amino acids 626-644 of human MMP-9
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	AB13458 recognizes proteins of 92kDa and ~86kDa which are identified as pro (latent) and activated forms of matrix metalloproteinase 9 (MMP-9; also known as 92kDa collagenase IV or gelatinase B). CHEMICON AB13458 shows no cross-reaction with pro and active forms of other MMPs. CELLULAR LOCALIZATION:Enzyme is localized within the cytoplasm.
Species Reactivity	Guinea Pig Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004994.2
Entrez Gene Summary	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Gene Symbol	MMP9 MMP-9 GELB CLG4B
UniProt Number	P14780
UniProt Summary	FUNCTION: SwissProt: P14780 # May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit. SIZE: 707 amino acids; 78427 Da SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes. DOMAIN: SwissProt: P14780 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains. MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.

Biological Information

Immunogen

Amino acids 626-644 of human MMP-9

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

AB13458 recognizes proteins of 92kDa and ~86kDa which are identified as pro (latent) and activated forms of matrix metalloproteinase 9 (MMP-9; also known as 92kDa collagenase IV or gelatinase B). CHEMICON AB13458 shows no cross-reaction with pro and active forms of other MMPs.

CELLULAR LOCALIZATION:Enzyme is localized within the cytoplasm.

Species Reactivity

Guinea Pig
Human

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_004994.2

Entrez Gene Summary

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Gene Symbol

MMP9
MMP-9
GELB
CLG4B

UniProt Number

P14780

UniProt Summary

FUNCTION: SwissProt: P14780 # May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit.
SIZE: 707 amino acids; 78427 Da
SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.
TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.
DOMAIN: SwissProt: P14780 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.
MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Antibody is stable for 12 months after date of receipt when stored at 2-8ºC.

Storage and Shipping Information

Storage Conditions

Antibody is stable for 12 months after date of receipt when stored at 2-8ºC.

Packaging Information
Material Size	500 µL

Packaging Information

Material Size

500 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-MMP-9, clone EP1254, Rabbit Monoclonal

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