Anti-GluR-2, C-terminus (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, M	WB	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

R, M

Serum

Polyclonal Antibody

Description
Catalogue Number	AB10529
Description	Anti-GluR-2 Antibody, CT
Alternate Names	glutamate receptor, ionotropic, AMPA 2 glutamate receptor 2 Glutamate receptor ionotropic, AMPA 2 AMPA-selective glutamate receptor 2
Background Information	Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Description

Catalogue Number

AB10529

Description

Anti-GluR-2 Antibody, CT

Alternate Names

glutamate receptor, ionotropic, AMPA 2
glutamate receptor 2
Glutamate receptor ionotropic, AMPA 2
AMPA-selective glutamate receptor 2

Background Information

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Product Information
Format	Serum
Control	PC12 cell lysate
Presentation	Rabbit polyclonal serum containing 0.05% sodium azide.

Product Information

Format

Serum

Control

PC12 cell lysate

Presentation

Rabbit polyclonal serum containing 0.05% sodium azide.

Applications
Application	Anti-GluR-2 Antibody, C-terminus detects level of GluR-2 & has been published & validated for use in WB.
Key Applications	Western Blotting
Application Notes	Immunoprecipitation: A previous lot of this antibody successfully immoprecipitated GluR-2 from a brain tissue lysate, as reported by an independent laboratory.

Applications

Application

Anti-GluR-2 Antibody, C-terminus detects level of GluR-2 & has been published & validated for use in WB.

Key Applications

Western Blotting

Application Notes

Immunoprecipitation: A previous lot of this antibody successfully immoprecipitated GluR-2 from a brain tissue lysate, as reported by an independent laboratory.

Biological Information
Immunogen	Recombinant protein corresponding to the C-terminus of rat GluR-2.
Epitope	C-terminus
Host	Rabbit
Specificity	This antibody recognizes GluR-2 at the C-terminus.
Species Reactivity	Rat Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001077280
Entrez Gene Summary	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq].
Gene Symbol	HBGR2 GLUR2 GLURB GluR-K2 GluR-B GluR-2 gluR-B GRIA2
Purification Method	Unpurified
UniProt Number	P19491
UniProt Summary	FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. SIZE: 883 amino acids; 98,688 Da SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4(By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression. TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells. SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
Molecular Weight	~106 kDa Observed

Biological Information

Immunogen

Recombinant protein corresponding to the C-terminus of rat GluR-2.

Epitope

C-terminus

Host

Rabbit

Specificity

This antibody recognizes GluR-2 at the C-terminus.

Species Reactivity

Rat
Mouse

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001077280

Entrez Gene Summary

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq].

Gene Symbol

HBGR2
GLUR2
GLURB
GluR-K2
GluR-B
GluR-2
gluR-B
GRIA2

Purification Method

Unpurified

UniProt Number

P19491

UniProt Summary

FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

SIZE: 883 amino acids; 98,688 Da

SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4(By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB.

SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression.

TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells.

SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.

Molecular Weight

~106 kDa Observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in PC12 cell lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected GluR-2 on 10 µg of PC12 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected GluR-2 on 10 µg of PC12 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-GluR-2, C-terminus (rabbit polyclonal)

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