Anti-TLS, clone 1FU-1D2 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, ICC	M	Ascites	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, ICC

Ascites

Monoclonal Antibody

Description
Catalogue Number	04-1552
Description	Anti-TLS Antibody, clone 1FU-1D2
Alternate Names	75 kDa DNA-pairing protein FUS-CHOP fusion protein FUS-CHOP protein fusion Fusion gene in myxoid liposarcoma Oncogene FUS Oncogene TLS Translocated in liposarcoma protein fus-like protein fus/tls-chop oncogene fusion (involved in t(12 16) in malignant liposarcoma) fusion, derived from t(12 16) malignant liposarcoma heterogeneous nuclear ribonucleoprotein P2 translocated in liposarcoma
Background Information	The TLS/FUS protein contains an RNA-recognition motif and is a component of nuclear riboprotein complexes. It resembles a transcription factor in that it binds DNA, contributes a transcriptional activation domain to the FUS-ERG oncoprotein, and interacts with several transcription factors in vitro. It binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. It may play a role in maintenance of genomic integrity.

Description

Catalogue Number

04-1552

Description

Anti-TLS Antibody, clone 1FU-1D2

Alternate Names

75 kDa DNA-pairing protein
FUS-CHOP fusion protein
FUS-CHOP protein fusion
Fusion gene in myxoid liposarcoma
Oncogene FUS
Oncogene TLS
Translocated in liposarcoma protein
fus-like protein
fus/tls-chop oncogene
fusion (involved in t(12
16) in malignant liposarcoma)
fusion, derived from t(12
16) malignant liposarcoma
heterogeneous nuclear ribonucleoprotein P2
translocated in liposarcoma

Background Information

The TLS/FUS protein contains an RNA-recognition motif and is a component of nuclear riboprotein complexes. It resembles a transcription factor in that it binds DNA, contributes a transcriptional activation domain to the FUS-ERG oncoprotein, and interacts with several transcription factors in vitro. It binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. It may play a role in maintenance of genomic integrity.

Product Information
Format	Ascites
Control	HepG2 cell lysate
Presentation	Unpurified mouse monoclonal IgG1κ preservative-free ascites.

Product Information

Format

Ascites

Control

HepG2 cell lysate

Presentation

Unpurified mouse monoclonal IgG1κ preservative-free ascites.

Applications
Application	Use Anti-TLS Antibody, clone 1FU-1D2 (Mouse Monoclonal Antibody) validated in WB, ICC to detect TLS also known as 75 kDa DNA-pairing protein, FUS-CHOP fusion protein.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected TLS in C2C12 cells.

Applications

Application

Use Anti-TLS Antibody, clone 1FU-1D2 (Mouse Monoclonal Antibody) validated in WB, ICC to detect TLS also known as 75 kDa DNA-pairing protein, FUS-CHOP fusion protein.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected TLS in C2C12 cells.

Biological Information
Immunogen	Linear peptide corresponding to human TLS.
Epitope	Unknown
Clone	clone 1FU-1D2
Host	Mouse
Specificity	This antibody recognizes TLS.
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_004951.1
Entrez Gene Summary	This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq].
Gene Symbol	FUS CHOP FUS-CHOP FUS1 POMp75 TLS TLS/CHOP hnRNP-P2
Purification Method	Unpurified
UniProt Number	P35637
UniProt Summary	FUNCTION: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. SUBUNIT STRUCTURE: Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. SUBCELLULAR LOCATION: Nucleus TISSUE SPECIFICITY: Ubiquitous. PTM: Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine. INVOLVEMENT IN DISEASE: A chromosomal aberration involving FUS is a cause of a form of malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3. A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG. A chromosomal aberration involving FUS is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein. Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%. SEQUENCE SIMILARITIES: Belongs to the RRM TET family. Contains 1 RanBP2-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
Molecular Weight	Observed at 65 kDa

Biological Information

Immunogen

Linear peptide corresponding to human TLS.

Epitope

Unknown

Clone

clone 1FU-1D2

Host

Mouse

Specificity

This antibody recognizes TLS.

Isotype

IgG1κ

Species Reactivity

Human
Mouse
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_004951.1

Entrez Gene Summary

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq].

Gene Symbol

FUS
CHOP
FUS-CHOP
FUS1
POMp75
TLS
TLS/CHOP
hnRNP-P2

Purification Method

Unpurified

UniProt Number

P35637

UniProt Summary

FUNCTION: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.

SUBUNIT STRUCTURE: Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP.

SUBCELLULAR LOCATION: Nucleus

TISSUE SPECIFICITY: Ubiquitous.

PTM: Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.

INVOLVEMENT IN DISEASE: A chromosomal aberration involving FUS is a cause of a form of malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.

A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.

A chromosomal aberration involving FUS is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.

Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.

SEQUENCE SIMILARITIES: Belongs to the RRM TET family.

Contains 1 RanBP2-type zinc finger.

Contains 1 RRM (RNA recognition motif) domain.

Molecular Weight

Observed at 65 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HepG2 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected TLS on 10 µg of HepG2 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected TLS on 10 µg of HepG2 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-TLS, clone 1FU-1D2 (mouse monoclonal)

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