Anti-VEGF, clone JH121 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Rb	IH(P)	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R, Rb

IH(P)

Purified

Monoclonal Antibody

Description
Catalogue Number	05-443
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-VEGF Antibody, clone JH
Alternate Names	Vascular permeability factor vascular endothelial growth factor vascular endothelial growth factor A vascular endothelial growth factor isoform VEGF165
Background Information	The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.

Description

Catalogue Number

05-443

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-VEGF Antibody, clone JH

Alternate Names

Vascular permeability factor
vascular endothelial growth factor
vascular endothelial growth factor A
vascular endothelial growth factor isoform VEGF165

Background Information

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.

Product Information
Format	Purified
Control	Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.
Presentation	Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.

Product Information

Format

Purified

Control

Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.

Presentation

Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.

Applications
Application	This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF.
Key Applications	Immunohistochemistry (Paraffin)
Applications Not Recommended	Western Blotting
Application Notes	Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C. Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.

Applications

Application

This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF.

Key Applications

Immunohistochemistry (Paraffin)

Applications Not Recommended

Western Blotting

Application Notes

Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C.

Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.

Biological Information
Immunogen	Recombinant human VEGF121. Clone JH121
Clone	clone JH
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat Rabbit
Species Reactivity Note	Human, rabbit, mouse, and rat. Other species not tested.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_003376
Entrez Gene Summary	This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
Gene Symbol	VEGFA VEGF-A VEGF VPF MGC70609
Purification Method	Protein G Chromatography
UniProt Number	P15692
UniProt Summary	FUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. SIZE: 232 amino acids; 27042 Da SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity). SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon. SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.
Molecular Weight	34-54 kDa isoforms

Biological Information

Immunogen

Recombinant human VEGF121. Clone JH121

Clone

clone JH

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).

Isotype

IgG1κ

Species Reactivity

Human
Mouse
Rat
Rabbit

Species Reactivity Note

Human, rabbit, mouse, and rat. Other species not tested.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_003376

Entrez Gene Summary

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

Gene Symbol

VEGFA
VEGF-A
VEGF
VPF
MGC70609

Purification Method

Protein G Chromatography

UniProt Number

P15692

UniProt Summary

FUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
SIZE: 232 amino acids; 27042 Da
SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.

Molecular Weight

34-54 kDa isoforms

Product Usage Statements
Quality Assurance	Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-VEGF, clone JH121 (mouse monoclonal)

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询