Anti-AGS3/GPSM1 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, M	WB, IF, IH(P)	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

R, M

WB, IF, IH(P)

Serum

Polyclonal Antibody

Description
Catalogue Number	ABS1531
Description	Anti-AGS3/GPSM1 Antibody
Alternate Names	G-protein-signaling modulator 1 Activator of G-protein signaling 3
Background Information	G-protein-signaling modulator 1 (UniProt Q9R080; also known as Activator of G-protein signaling 3) is encoded by the Gpsm1 (also known as Ags3) gene (Gene ID 246254) in rat species. AGS3/GPSM1 is an evolutionarily conserved protein with four G-protein regulatory (GPR) GoLoco motifs (a.a. 493-515, 546-568, 594-616, 628-650 of murine GPSM1) and functions as a guanine nucleotide dissociation inhibitor (GDI). GPSM1 increases heteromeric polycystin-1/polycystin-2 ion channel activity via Gβγ subunits. GPSM1 is involved in regulating mitotic spindle orientation, cell polarity, and adenylyl cyclase activity. GPSM1 plays a role in renal epithelial cell repair following renal injury and GPSM1 induction in polycystic kidney disease (PKD) and may be a critical modulator of the renal cystogenic process.

Description

Catalogue Number

ABS1531

Description

Anti-AGS3/GPSM1 Antibody

Alternate Names

G-protein-signaling modulator 1
Activator of G-protein signaling 3

Background Information

G-protein-signaling modulator 1 (UniProt Q9R080; also known as Activator of G-protein signaling 3) is encoded by the Gpsm1 (also known as Ags3) gene (Gene ID 246254) in rat species. AGS3/GPSM1 is an evolutionarily conserved protein with four G-protein regulatory (GPR) GoLoco motifs (a.a. 493-515, 546-568, 594-616, 628-650 of murine GPSM1) and functions as a guanine nucleotide dissociation inhibitor (GDI). GPSM1 increases heteromeric polycystin-1/polycystin-2 ion channel activity via Gβγ subunits. GPSM1 is involved in regulating mitotic spindle orientation, cell polarity, and adenylyl cyclase activity. GPSM1 plays a role in renal epithelial cell repair following renal injury and GPSM1 induction in polycystic kidney disease (PKD) and may be a critical modulator of the renal cystogenic process.

Product Information
Format	Serum
Presentation	Rabbit polyclonal antibody serum with 0.05% sodium azide.

Product Information

Format

Serum

Presentation

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Applications
Application	This Anti-AGS3 Antibody is validated for use in Western Blotting, Immunofluorescence, Immunohistochemistry (Paraffin) for the detection of GPSM1.
Key Applications	Western Blotting Immunofluorescence Immunohistochemistry (Paraffin)
Application Notes	Immunofluorescence Analysis: A representative lot immunostained predominantly renal collecting ducts by fluorescent immunohistochemistry using kidney sections from postnatal P12 Pkd1v/v ADPKD mice (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467). Immunohistochemistry Analysis: A representative lot detected a time-dependent induction of AGS3/GPSM1 immunoreactivity co-localized with that of the proliferation marker Ki-67 in formalin-fixed, paraffin-embedded kidney sections from rats following ischemia-reperfusion injury (IRI) initiated by a 30-min occlusion of renal blood flow via bilateral renal artery clamping (Regner, K.R., et al. (2011). FASEB J. 25(6):1844-1855). Western Blotting Analysis: A representative lot detected developmental stage-dependent renal AGS3/GPSM1 expression level. High level was seen in E13.5 embryonic kidney and greatly reduced among postnatal mice with low-to-undetectable levels around postnatal day P28. Postnatal renal AGS3/GPSM1 levels were found elevated and remained detectable up to P28 among Pkd1v/v autosomal dominant polycystic kidney disease (ADPKD) mice expressing noncleavable polycystin-1/PC1 (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467). Western Blotting Analysis: A representative lot detected much higher AGS3/GPSM1 expression level in brain than kidney from postnatal day 19 (P19) wild-type or Pkd1v/v ADPKD mice (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467). Western Blotting Analysis: A representative lot detected a time-dependent renal AGS3/GPSM1 induction in rats following ischemia-reperfusion injury (IRI) initiated by a 40-min occlusion of renal blood flow (Regner, K.R., et al. (2011). FASEB J. 25(6):1844-1855).

Applications

Application

This Anti-AGS3 Antibody is validated for use in Western Blotting, Immunofluorescence, Immunohistochemistry (Paraffin) for the detection of GPSM1.

Key Applications

Western Blotting
Immunofluorescence
Immunohistochemistry (Paraffin)

Application Notes

Immunofluorescence Analysis: A representative lot immunostained predominantly renal collecting ducts by fluorescent immunohistochemistry using kidney sections from postnatal P12 Pkd1v/v ADPKD mice (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467).
Immunohistochemistry Analysis: A representative lot detected a time-dependent induction of AGS3/GPSM1 immunoreactivity co-localized with that of the proliferation marker Ki-67 in formalin-fixed, paraffin-embedded kidney sections from rats following ischemia-reperfusion injury (IRI) initiated by a 30-min occlusion of renal blood flow via bilateral renal artery clamping (Regner, K.R., et al. (2011). FASEB J. 25(6):1844-1855).
Western Blotting Analysis: A representative lot detected developmental stage-dependent renal AGS3/GPSM1 expression level. High level was seen in E13.5 embryonic kidney and greatly reduced among postnatal mice with low-to-undetectable levels around postnatal day P28. Postnatal renal AGS3/GPSM1 levels were found elevated and remained detectable up to P28 among Pkd1v/v autosomal dominant polycystic kidney disease (ADPKD) mice expressing noncleavable polycystin-1/PC1 (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467).
Western Blotting Analysis: A representative lot detected much higher AGS3/GPSM1 expression level in brain than kidney from postnatal day 19 (P19) wild-type or Pkd1v/v ADPKD mice (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467).
Western Blotting Analysis: A representative lot detected a time-dependent renal AGS3/GPSM1 induction in rats following ischemia-reperfusion injury (IRI) initiated by a 40-min occlusion of renal blood flow (Regner, K.R., et al. (2011). FASEB J. 25(6):1844-1855).

Biological Information
Immunogen	Linear peptide corresponding to a sequence within the STK11/LKB1-interacting region of rat AGS3/GPSM1.
Epitope	STK11/LKB1-interacting region.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	This polyclonal antiserum immunostained predominantly renal collecting ducts of postnatal P12 ADPKD mice. Immunogen peptide blocking eliminated antibody stianing (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467). This antiserum targets a STK11/LKB1-interacting region sequence present in rat AGS3/GPSM1 isoform 1 and isoform 4 (isoform Long), 28% present in isoform 3 (Short-2), but completely absent from isoforms 2 (Short-1) reported by UniProt (Q9R080). Target sequence is also present in mouse isoforms 1, 3, 4, and 5, but absent from isoform 2 ( (UniProt Q6IR34).
Species Reactivity	Rat Mouse
Species Reactivity Note	Rat, Mouse.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_653346
Gene Symbol	Gpsm1 Ags3
Purification Method	Unpurified.
UniProt Number	Q9R080
Molecular Weight	~74/25 kDa oberved. 74.44 kDa (rat isoform 1), 72.05 kDa (rat isoform 4; isoform Long), and 25.08 kDa (rat isoform 3; isoform Short-2), 74.36/71.93/33.56/78.11 kDa (mouse isoform 1/3/4/5) calculated. Uncharacterized band(s) may appear in some lysates.

Biological Information

Immunogen

Linear peptide corresponding to a sequence within the STK11/LKB1-interacting region of rat AGS3/GPSM1.

Epitope

STK11/LKB1-interacting region.

Concentration

Please refer to lot specific datasheet.

Host

Rabbit

Specificity

This polyclonal antiserum immunostained predominantly renal collecting ducts of postnatal P12 ADPKD mice. Immunogen peptide blocking eliminated antibody stianing (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467). This antiserum targets a STK11/LKB1-interacting region sequence present in rat AGS3/GPSM1 isoform 1 and isoform 4 (isoform Long), 28% present in isoform 3 (Short-2), but completely absent from isoforms 2 (Short-1) reported by UniProt (Q9R080). Target sequence is also present in mouse isoforms 1, 3, 4, and 5, but absent from isoform 2 ( (UniProt Q6IR34).

Species Reactivity

Rat
Mouse

Species Reactivity Note

Rat, Mouse.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_653346

Gene Symbol

Gpsm1
Ags3

Purification Method

Unpurified.

UniProt Number

Q9R080

Molecular Weight

~74/25 kDa oberved. 74.44 kDa (rat isoform 1), 72.05 kDa (rat isoform 4; isoform Long), and 25.08 kDa (rat isoform 3; isoform Short-2), 74.36/71.93/33.56/78.11 kDa (mouse isoform 1/3/4/5) calculated. Uncharacterized band(s) may appear in some lysates.

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in embryonic mouse brain tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected AGS3/GPSM1 in 10 µg of E13-14 embryonic mouse brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in embryonic mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected AGS3/GPSM1 in 10 µg of E13-14 embryonic mouse brain tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-AGS3/GPSM1 (rabbit polyclonal)

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