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Ms Anti-Cyclin A

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货号：MAB3682

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, H, M, Mi IP, WB M Ascites Monoclonal Antibody
Description
Catalogue Number MAB3682
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Cyclin A Antibody, clone E67.1
References
Product Information
Format Ascites
Presentation Ascites fluid. Liquid. Contains no preservative.
Applications
Application Anti-Cyclin A Antibody, clone E67.1 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin A & has been validated in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application Notes Western blot

Immunoprecipitation

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Bovine Cyclin A.
Clone E67.1
Host Mouse
Specificity Cyclin A. MAB3682 is able to immunoprecipitate active cdk/cyclin A H1 kinase from a wide variety of species. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A is a marker for actively proliferating cells and for cells in S phase.
Isotype IgG2a
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Mink
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • CCNA1
  • Cyclin-A1.
UniProt Number
UniProt Summary FUNCTION: SwissProt: P78396 # May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
SIZE: 465 amino acids; 52358 Da
SUBUNIT: Interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Does not bind CDK4 and CDK5 (in vitro). The cyclin A1-CDK2 complex interacts with transcription factor E2F-1 and RB proteins. Found in a complex with CDK2, CABLES1 and CCNE1 (By similarity). Interacts with KLHDC9.
TISSUE SPECIFICITY: Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
DEVELOPMENTAL STAGE: Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
SIMILARITY: SwissProt: P78396 ## Belongs to the cyclin family. Cyclin AB subfamily.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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