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Ms X Human Cdc20

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货号：MAB3775

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IF, IP M Purified Monoclonal Antibody
Description
Catalogue Number MAB3775
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Cdc20 Antibody, clone AR12
Alternate Names
  • Cell Division Cycle Protein 20 Homolog
  • p55cdc
References
Product Information
Format Purified
Presentation Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.
Applications
Application Anti-Cdc20 Antibody, clone AR12 is a Mouse Monoclonal Antibody for detection of Cdc20 also known as Cell Division Cycle Protein 20 Homolog p55cdc & has been validated in IF & IP.
Key Applications
  • Immunofluorescence
  • Immunoprecipitation
Application Notes Immunofluorescence

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Human Cdc20 recombinant protein
Clone AR12
Host Mouse
Specificity Recognizes human Cdc20
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.
Gene Symbol
  • CDC20
  • CDC20A
  • MGC102824
  • p55CDC
  • P55CDC-LSB
  • bA276H19.3
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q12834 # Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
SIZE: 499 amino acids; 54723 Da
SUBUNIT: Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1 (By similarity). Interacts with MAD2L1. The phosphorylated form interacts with APC/C.
DEVELOPMENTAL STAGE: Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
PTM: Phosphorylated during mitosis, probably by maturation promoting factor (MPF).
SIMILARITY: SwissProt: Q12834 ## Belongs to the WD repeat CDC20/Fizzy family. & Contains 7 WD repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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