Anti-Fibroblast activation protein alpha (FAP) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IHC, FUNC	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, IHC, FUNC

Purified

Polyclonal Antibody

Description
Catalogue Number	ABT11
Description	Anti-Fibroblast activation protein alpha (FAP) Antibody
Alternate Names	fibroblast activation protein, alpha fibroblast activation protein, alpha subunit integral membrane serine protease seprase 170 kDa melanoma membrane-bound gelatinase, FAP
Background Information	Fibroblast activation protein alpha (FAP), also known as Seprase, is a 97 kDa, type II cell surface glycoprotein belonging to the serine protease family. Although FAP is considered fibroblast specific, it is not found in normal healthy fibroblasts. FAP is most commonly found in stromal, tumor fibroblasts, and is likely involved in ECM pericellular proteolysis, endothelial cell migration and invasion into the ECM. The presence of FAP in human epithelial carcinoma is increased, particularly in breast, colorectal, lung, and ovarian carcinomas. FAP is also transiently observed in developing cells, or in tissues that are undergoing wound healing or suffering from chronic inflammatory conditions. Due to its specific distribution both to tumor fibroblasts and to inflammed tissue, FAP makes a promising target for research in tumor-stromal studies and in therapeutic studies.

Description

Catalogue Number

ABT11

Description

Anti-Fibroblast activation protein alpha (FAP) Antibody

Alternate Names

fibroblast activation protein, alpha
fibroblast activation protein, alpha subunit
integral membrane serine protease
seprase
170 kDa melanoma membrane-bound gelatinase, FAP

Background Information

Fibroblast activation protein alpha (FAP), also known as Seprase, is a 97 kDa, type II cell surface glycoprotein belonging to the serine protease family. Although FAP is considered fibroblast specific, it is not found in normal healthy fibroblasts. FAP is most commonly found in stromal, tumor fibroblasts, and is likely involved in ECM pericellular proteolysis, endothelial cell migration and invasion into the ECM. The presence of FAP in human epithelial carcinoma is increased, particularly in breast, colorectal, lung, and ovarian carcinomas. FAP is also transiently observed in developing cells, or in tissues that are undergoing wound healing or suffering from chronic inflammatory conditions. Due to its specific distribution both to tumor fibroblasts and to inflammed tissue, FAP makes a promising target for research in tumor-stromal studies and in therapeutic studies.

Product Information
Format	Purified
Control	HEK-FAP xenograft tumor lysate
Presentation	Purified rabbit polyclonal in buffer containing PBS (pH 7.2) with 0.05% sodium azide.

Product Information

Format

Purified

Control

HEK-FAP xenograft tumor lysate

Presentation

Purified rabbit polyclonal in buffer containing PBS (pH 7.2) with 0.05% sodium azide.

Applications
Application	Detect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.
Key Applications	Western Blotting Immunohistochemistry Affects Function
Application Notes	Immunohistochemistry: A 1:750 dilution of a previous lot of FAP antibody was used to immunohistochemically stain frozen tissue sections of HEK-FAP or HT-29 xenograft tumors from C.B17 mice (Cheng, 2002). Inhibition: Inhibition of dipeptidyl peptidase activity with a previous lot of FAP antibody on HT-29 xenograft tumors was demonstrated by an independent laboratory (Cheng, 2002).

Applications

Application

Detect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.

Key Applications

Western Blotting
Immunohistochemistry
Affects Function

Application Notes

Immunohistochemistry: A 1:750 dilution of a previous lot of FAP antibody was used to immunohistochemically stain frozen tissue sections of HEK-FAP or HT-29 xenograft tumors from C.B17 mice (Cheng, 2002).

Inhibition: Inhibition of dipeptidyl peptidase activity with a previous lot of FAP antibody on HT-29 xenograft tumors was demonstrated by an independent laboratory (Cheng, 2002).

Biological Information
Immunogen	Histidine-tagged recombinant protein corresponding to human FAP.
Epitope	Extracellular domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	The antibody recognizes the extracellular domain of Fibroblast activation protein alpha
Isotype	IgG
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_004451
Entrez Gene Summary	The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [provided by RefSeq].
Gene Symbol	FAPA FAP DPPIV
Purification Method	Ammonium Sulfate
UniProt Number	Q12884
UniProt Summary	FUNCTION: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. CATALYTIC ACTIVITY: Degrades gelatin and heat-denatured type I and type IV collagen, but not native type I or type IV collagen. Does not cleave laminin, fibronectin, fibrin or casein. SUBUNIT STRUCTURE: Homodimer, or heterodimer with DPP4. The monomer is inactive. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. Cell projection › lamellipodium membrane; Single-pass type II membrane protein. Cell projection › invadopodium membrane; Single-pass type II membrane protein. Note: Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. TISSUE SPECIFICTY: Fibroblast specific. INDUCTION: In fibroblasts at times and sites of tissue remodeling during development, tissue repair, and carcinogenesis. PTM: N-glycosylated. Ref.11 Ref.9 The N-terminus may be blocked. SEQUENCE SIMILARITIES: Belongs to the peptidase S9B family.
Molecular Weight	~ 90 kDa observed MW. Two isoforms of the Fibroblast activation protein exist at calculated MWs of 88kDa and 27 kDa. It is possible that both bands may be obserbved in certain cell lines.

Biological Information

Immunogen

Histidine-tagged recombinant protein corresponding to human FAP.

Epitope

Extracellular domain

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

The antibody recognizes the extracellular domain of Fibroblast activation protein alpha

Isotype

IgG

Species Reactivity

Human

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_004451

Entrez Gene Summary

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [provided by RefSeq].

Gene Symbol

FAPA
FAP
DPPIV

Purification Method

Ammonium Sulfate

UniProt Number

Q12884

UniProt Summary

FUNCTION: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

CATALYTIC ACTIVITY: Degrades gelatin and heat-denatured type I and type IV collagen, but not native type I or type IV collagen. Does not cleave laminin, fibronectin, fibrin or casein.

SUBUNIT STRUCTURE: Homodimer, or heterodimer with DPP4. The monomer is inactive.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. Cell projection › lamellipodium membrane; Single-pass type II membrane protein. Cell projection › invadopodium membrane; Single-pass type II membrane protein. Note: Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.

TISSUE SPECIFICTY: Fibroblast specific.

INDUCTION: In fibroblasts at times and sites of tissue remodeling during development, tissue repair, and carcinogenesis.

PTM: N-glycosylated. Ref.11 Ref.9

The N-terminus may be blocked.

SEQUENCE SIMILARITIES: Belongs to the peptidase S9B family.

Molecular Weight

~ 90 kDa observed MW. Two isoforms of the Fibroblast activation protein exist at calculated MWs of 88kDa and 27 kDa. It is possible that both bands may be obserbved in certain cell lines.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HEK-FAP xenograft tumor lysate. Western Blot Analysis: 2.5 µg/mL of this antibody detected FAP in 10 µg of HEK-FAP xenograft tumor lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HEK-FAP xenograft tumor lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected FAP in 10 µg of HEK-FAP xenograft tumor lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-Fibroblast activation protein alpha (FAP) (rabbit polyclonal)

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