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Anti-MAPKAP-K2, clone 7H4.2

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货号：04-539

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB M Purified Monoclonal Antibody
Description
Catalogue Number 04-539
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-MAPKAP-K2 Antibody, clone 7H4.2
Alternate Names
  • MAPK-activated protein kinase 2
  • MAPKAP kinase 2
References
Product Information
Format Purified
Presentation 200 µg (1 mg/mL) of mouse monoclonal IgG in PBS containing 0.1% sodium azide.
Applications
Application Anti-MAPKAP-K2 Antibody, clone 7H4.2 detects level of MAPKAP-K2 & has been published & validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application Notes Suggested dilutions:
Immunoblot Analysis: 1:500
Immunoprecipitation: 1:20
Biological Information
Immunogen KLH-conjugated synthetic peptide of the C-terminus of the long form of human MAPKAP-K2.
Clone 7H4.2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Gene Symbol
  • MAPKAPK2
  • MK2
  • MAPKAPK-2
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
SIZE: 400 amino acids; 45568 Da
SUBUNIT: Interacts with PHC2.
TISSUE SPECIFICITY: Expressed in all tissues examined.
PTM: Phosphorylated and activated by MAP kinase.
SIMILARITY: SwissProt: P49137 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
Molecular Weight 45 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C from date of shipment.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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