Anti-phospho-MAP Kinase (Erk1/2), clone 12D4 monoclonal IgG1k

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Av, Ca	WB, IP, ELISA, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R, Av, Ca

WB, IP, ELISA, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	05-481
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-MAP Kinase1/2 Antibody
Alternate Names	Extracellular signal-regulated kinase Insulin-stimulated MAP2 kinase MAP kinase 1 MAPK 1 Microtubule-associated protein 2 kinase extracellular signal-related kinase 1 mitogen-activated protein kinase 3
Background Information	The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.

Description

Catalogue Number

05-481

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-phospho-MAP Kinase1/2 Antibody

Alternate Names

Extracellular signal-regulated kinase
Insulin-stimulated MAP2 kinase
MAP kinase 1
MAPK 1
Microtubule-associated protein 2 kinase
extracellular signal-related kinase 1
mitogen-activated protein kinase 3

Background Information

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.

Product Information
Format	Purified
Control	Included Positive Control Lysate: Catalog # 12-302, EGF-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg (20 μL) of reduced lysate per lane for minigels.
Presentation	Purified mouse monoclonal IgG1k lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL.

Product Information

Format

Purified

Control

Included Positive Control Lysate: Catalog # 12-302, EGF-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg (20 μL) of reduced lysate per lane for minigels.

Presentation

Purified mouse monoclonal IgG1k lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose.
Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL.

Applications
Application	Detect phospho-MAP Kinase1/2 using this Anti-phospho-MAP Kinase1/2 validated for use in WB, IP, ELISA & IC.
Key Applications	Western Blotting Immunoprecipitation ELISA Immunocytochemistry
Application Notes	Immunoprecipitation: Use 1-10 μg per immuno-precipitation.

Applications

Application

Detect phospho-MAP Kinase1/2 using this Anti-phospho-MAP Kinase1/2 validated for use in WB, IP, ELISA & IC.

Key Applications

Western Blotting
Immunoprecipitation
ELISA
Immunocytochemistry

Application Notes

Immunoprecipitation: Use 1-10 μg per immuno-precipitation.

Biological Information
Immunogen	KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4
Clone	clone 12D4
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Specific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2.
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat Avian Canine
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002745 NM_002746
Entrez Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. ...
Gene Symbol	MAPK3 MGC20180 ERT2 HUMKER1A p44-MAPK PRKM3 ERK1 p44erk1 p44mapk P44MAPK HS44KDAP p44-ERK1 P44ERK1 ERK-1
Modifications	Phosphorylation
Purification Method	Thiophilic adsoption and size exclusion chromatography
UniProt Number	P27361
UniProt Summary	FUNCTION: SwissProt: P27361 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). COFACTOR: Magnesium (By similarity). SIZE: 379 amino acids; 43136 Da SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4. DOMAIN: SwissProt: P27361 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ...
Molecular Weight	42-44 kDa

Biological Information

Immunogen

KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4

Clone

clone 12D4

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Specific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2.

Isotype

IgG1κ

Species Reactivity

Human
Mouse
Rat
Avian
Canine

Antibody Type

Monoclonal Antibody

Entrez Gene Number

Entrez Gene Summary

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. ...

Gene Symbol

MAPK3
MGC20180
ERT2
HUMKER1A
p44-MAPK
PRKM3
ERK1
p44erk1
p44mapk
P44MAPK
HS44KDAP
p44-ERK1
P44ERK1
ERK-1

Modifications

Phosphorylation

Purification Method

Thiophilic adsoption and size exclusion chromatography

UniProt Number

P27361

UniProt Summary

FUNCTION: SwissProt: P27361 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 379 amino acids; 43136 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P27361 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ...

Molecular Weight

42-44 kDa

Product Usage Statements
Quality Assurance	Evaluated by western blot on RIPA lysates from EGF-stimulated human A431 carcinoma cells. Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by western blot on RIPA lysates from EGF-stimulated human A431 carcinoma cells.

Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-phospho-MAP Kinase (Erk1/2), clone 12D4 monoclonal IgG1k

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