Anti-phospho STAT3 (Tyr705), clone 9E12

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, Ca	ELISA, IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, Ca

ELISA, IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-485
Replaces	04-1059
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12
Alternate Names	Acute-phase response factor DNA-binding protein APRF signal transducer and activator of transcription 3 signal transducer and activator of transcription 3 (acute-phase response factor)
Background Information	STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

Description

Catalogue Number

05-485

Replaces

04-1059

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

Alternate Names

Acute-phase response factor
DNA-binding protein APRF
signal transducer and activator of transcription 3
signal transducer and activator of transcription 3 (acute-phase response factor)

Background Information

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

Product Information
Format	Purified
Control	SK-N-MC cells murine macrophage 4/4 cells, serum-starved for 16 hours then stimulated with Phorbol 12-myristate 13-acetate (PMA) for 10 minutes
Presentation	Lyophilized purified mouse IgG1ќ from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.

Product Information

Format

Purified

Control

SK-N-MC cells
murine macrophage 4/4 cells, serum-starved for 16 hours then stimulated with Phorbol 12-myristate 13-acetate (PMA) for 10 minutes

Presentation

Lyophilized purified mouse IgG1ќ from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.

Applications
Application	Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 (Tyr705) also known as Acute-phase response factor or DNA-binding protein APRF. This Stat3 Antibody has been validated in Human, Mouse and Canine samples.
Key Applications	ELISA Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: An independent laboratory has reported 1-10 µg/mL of a previous lot of this antibody immunoprecipitated phospho-STAT3 (Tyr705) from a lysate of 106 vanadate-treated human A431 cells. ELISA: 0.05 µg/mL of this antibody is recommended by an independent lab for ELISA testing. Test was conducted on a previous lot.

Applications

Application

Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 (Tyr705) also known as Acute-phase response factor or DNA-binding protein APRF. This Stat3 Antibody has been validated in Human, Mouse and Canine samples.

Key Applications

ELISA
Immunoprecipitation
Western Blotting

Application Notes

Immunoprecipitation: An independent laboratory has reported 1-10 µg/mL of a previous lot of this antibody immunoprecipitated phospho-STAT3 (Tyr705) from a lysate of 106 vanadate-treated human A431 cells.

ELISA: 0.05 µg/mL of this antibody is recommended by an independent lab for ELISA testing. Test was conducted on a previous lot.

Biological Information
Immunogen	KLH-conjugated phosphopeptide containing the AP[pY]LK motif of STAT3
Clone	9E12
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes STAT3, Mr 92 kDa, phos-phorylated on tyrosine-705. Clone 9E12.
Isotype	IgG1κ
Species Reactivity	Human Mouse Canine
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_139276
Entrez Gene Summary	The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) lacks a segment in the 5' UTR and 3 nt within the CDS, as compared to variant 1. The resulting isoform (2) lacks an amino acid compared to isoform 1.
Gene Symbol	STAT3 APRF FLJ20882 MGC16063
Modifications	Phosphorylation
Purification Method	Thiophilic adsoption and size exclusion chromatography
UniProt Number	P40763
UniProt Summary	FUNCTION: SwissProt: P40763 # Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. SIZE: 770 amino acids; 88068 Da SUBUNIT: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with NCOA1, PELP1, SOCS7 and STATIP1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts with IL31RA. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. PTM: Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. DISEASE: "SwissProt: P40763 # Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures." SIMILARITY: SwissProt: P40763 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain. MISCELLANEOUS: Involved in the gp130-mediated signaling pathway." ...
Molecular Weight	92 kDa

Biological Information

Immunogen

KLH-conjugated phosphopeptide containing the AP[pY]LK motif of STAT3

Clone

9E12

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes STAT3, Mr 92 kDa, phos-phorylated on tyrosine-705. Clone 9E12.

Isotype

IgG1κ

Species Reactivity

Human
Mouse
Canine

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_139276

Entrez Gene Summary

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq].

Transcript Variant: This variant (2) lacks a segment in the 5' UTR and 3 nt within the CDS, as compared to variant 1. The resulting isoform (2) lacks an amino acid compared to isoform 1.

Gene Symbol

STAT3
APRF
FLJ20882
MGC16063

Modifications

Phosphorylation

Purification Method

Thiophilic adsoption and size exclusion chromatography

UniProt Number

P40763

UniProt Summary

FUNCTION: SwissProt: P40763 # Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
SIZE: 770 amino acids; 88068 Da
SUBUNIT: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with NCOA1, PELP1, SOCS7 and STATIP1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts with IL31RA.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.
TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PTM: Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity.
DISEASE: "SwissProt: P40763 # Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures."
SIMILARITY: SwissProt: P40763 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain.
MISCELLANEOUS: Involved in the gp130-mediated signaling pathway." ...

Molecular Weight

92 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by immunoblot on lysates from human SK-N-MC cells stimulated with CNTF. Western Blot Analysis: 0.1-1 µg/mL of this lot detected phospho-STAT3 (Tyr705) in lysates from human SK-NMC cells stimulated with CNTF.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by immunoblot on lysates from human SK-N-MC cells stimulated with CNTF.

Western Blot Analysis:
0.1-1 µg/mL of this lot detected phospho-STAT3 (Tyr705) in lysates from human SK-NMC cells stimulated with CNTF.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-phospho STAT3 (Tyr705), clone 9E12

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