Anti-IGF-1R, ß-subunit, clone 1-2 (mouse monoclonal)

Replacement Information
Replacement Information	05-1106 is a recommended replacement for MAB1123

Replacement Information

05-1106 is a recommended replacement for MAB1123

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M	WB, IH(P)	M	Affinity Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R, M

WB, IH(P)

Affinity Purified

Monoclonal Antibody

Description
Catalogue Number	05-1106
Replaces	MAB1123
Description	Anti-IGF-IR Antibody, β-subunit, clone 1-2
Background Information	IGF-I and -II signal through the IGF-I Receptor, which is homologous to the Insulin Receptor. The high-affinity IGF-II Receptor does not play a direct role in signaling, but regulates the concentration of free IGF-II. The IGFs are involved in skeletal growth, and are essential for prevention of apoptosis. Serum levels of free IGFs are kept low by the action of IGF binding proteins (IGFBPs), which sequester the IGFs. Overexpression of IGFBPs may induce apoptosis, presumably by reduction of free IGF; IGFBP levels are also altered in some cancers. The IGF-I Receptor is not as mitogenic as some other growth factor receptors, but its ability to activate the PI3 Kinase pathway, through the Insulin Receptor Substrate (IRS) proteins, is critical for mediating cell survival.

Description

Catalogue Number

05-1106

Replaces

MAB1123

Description

Anti-IGF-IR Antibody, β-subunit, clone 1-2

Background Information

IGF-I and -II signal through the IGF-I Receptor, which is homologous to the Insulin Receptor. The high-affinity IGF-II Receptor does not play a direct role in signaling, but regulates the concentration of free IGF-II. The IGFs are involved in skeletal growth, and are essential for prevention of apoptosis. Serum levels of free IGFs are kept low by the action of IGF binding proteins (IGFBPs), which sequester the IGFs. Overexpression of IGFBPs may induce apoptosis, presumably by reduction of free IGF; IGFBP levels are also altered in some cancers. The IGF-I Receptor is not as mitogenic as some other growth factor receptors, but its ability to activate the PI3 Kinase pathway, through the Insulin Receptor Substrate (IRS) proteins, is critical for mediating cell survival.

Product Information
Format	Affinity Purified
Control	C2C12 Cell Lysate
Presentation	Purified mouse monoclonal IgG2b in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Product Information

Format

Affinity Purified

Control

C2C12 Cell Lysate

Presentation

Purified mouse monoclonal IgG2b in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Applications
Application	Anti-IGF-IR Antibody, β-subunit, clone 1-2 is an antibody against IGF-IR for use in WB, IH(P).
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of IGF Monoclonal Antibody: Placenta Western Blot: A previous lot of this antibody was used at 1-2 µg/mL for 2 hours at room temperature. Optimal working dilutions must be determined by end user.

Applications

Application

Anti-IGF-IR Antibody, β-subunit, clone 1-2 is an antibody against IGF-IR for use in WB, IH(P).

Key Applications

Western Blotting
Immunohistochemistry (Paraffin)

Application Notes

Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of IGF Monoclonal Antibody: Placenta

Western Blot: A previous lot of this antibody was used at 1-2 µg/mL for 2 hours at room temperature.

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	A 15-mer peptide corresponding to a.a. 1323-1337 (Tyr-RKNERALPLPQSSTC) from the C-terminal of β-subunit of human IGF-1R.
Epitope	Between a.a. 1323-1337 of IGF-1R
Clone	1-2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes the β-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 1323-1337 of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the α- and β-subunits, which are disulfide-linked in a β-α-α-β configuration in the mature receptor. The α-subunit is completely extracellular, while the β-subunit spans membrane.
Isotype	IgG2b
Species Reactivity	Human Rat Mouse
Species Reactivity Note	Mouse and rat. Expected to react with human based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000875.3
Entrez Gene Summary	This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq]
Gene Symbol	IGF1R MGC142170 MGC18216 IGFIR JTK13 MGC142172 CD221
Purification Method	Affinity Purfied
UniProt Number	P08069
UniProt Summary	FUNCTION: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. ENZYME REGULATION: Autophosphorylation activates the kinase activity. SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed in a variety of tissues. PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I). PTM: Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding. DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 3 fibronectin type-III domains. SIMILARITY: Contains 1 protein kinase domain.
Molecular Weight	Recognizes the beta-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R; ~140 kDa)

Biological Information

Immunogen

A 15-mer peptide corresponding to a.a. 1323-1337 (Tyr-RKNERALPLPQSSTC) from the C-terminal of β-subunit of human IGF-1R.

Epitope

Between a.a. 1323-1337 of IGF-1R

Clone

1-2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes the β-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 1323-1337 of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the α- and β-subunits, which are disulfide-linked in a β-α-α-β configuration in the mature receptor. The α-subunit is completely extracellular, while the β-subunit spans membrane.

Isotype

IgG2b

Species Reactivity

Human
Rat
Mouse

Species Reactivity Note

Mouse and rat. Expected to react with human based on sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000875.3

Entrez Gene Summary

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq]

Gene Symbol

IGF1R
MGC142170
MGC18216
IGFIR
JTK13
MGC142172
CD221

Purification Method

Affinity Purfied

UniProt Number

P08069

UniProt Summary

FUNCTION: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
ENZYME REGULATION: Autophosphorylation activates the kinase activity.
SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
PTM: Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
SIMILARITY: Contains 3 fibronectin type-III domains.
SIMILARITY: Contains 1 protein kinase domain.

Molecular Weight

Recognizes the beta-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R; ~140 kDa)

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on C2C12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected IGF-1 Receptor beta on 10 μg of C2C12 lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected IGF-1 Receptor beta on 10 μg of C2C12 lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-IGF-1R, ß-subunit, clone 1-2 (mouse monoclonal)

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