Anti-PP2A, B b subunit (rabbit polyclonal)

Replacement Information
Replacement Information	07-1219 is a recommended replacement for AB4086

Replacement Information

07-1219 is a recommended replacement for AB4086

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, H, M, R	WB, IH(P)	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

B, H, M, R

WB, IH(P)

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-1219
Replaces	AB4086
Description	Anti-PP2A Antibody, B β subunit
Alternate Names	Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform PP2A-beta
Background Information	PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.

Description

Catalogue Number

07-1219

Replaces

AB4086

Description

Anti-PP2A Antibody, B β subunit

Alternate Names

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
PP2A-beta

Background Information

PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.

Product Information
Format	Affinity Purified
Control	NIH/3T3 Cell Lysate
Presentation	Purified rabbit polyclonal IgG provided as 0.2 µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Product Information

Format

Affinity Purified

Control

NIH/3T3 Cell Lysate

Presentation

Purified rabbit polyclonal IgG provided as 0.2 µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Applications
Application	Anti-PP2A Antibody, B β subunit detects level of PP2A & has been published & validated for use in WB, IH(P).
Key Applications	Western Blotting Immunohistochemistry (Paraffin)

Applications

Application

Anti-PP2A Antibody, B β subunit detects level of PP2A & has been published & validated for use in WB, IH(P).

Key Applications

Western Blotting
Immunohistochemistry (Paraffin)

Biological Information
Immunogen	The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Gly-Glu-Glu-Asp-Ile-Asp-THr-Arg- Lys-Ile-Asn-Asn-Ser-Phe-COOH.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Protein Phosphatase 2A/B. Minimal cross reactivity against 2A/B.
Isotype	IgG
Species Reactivity	Bovine Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001009552
Entrez Gene Summary	The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in the 5' UTR of this gene may cause a rare form of autosomal dominant spinocerebellar ataxia 12.
Gene Symbol	PPP2CB PP2CB PP2A-beta EC 3.1.3.16
Purification Method	Affinity Purfied
UniProt Number	P62714
UniProt Summary	FUNCTION: SwissProt: Q00005 # The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. SIZE: 443 amino acids; 51710 Da SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. TISSUE SPECIFICITY: Brain. DISEASE: SwissProt: Q00005 # Defects in PPP2R2B are the cause of spinocerebellar ataxia 12 (SCA12) [MIM:604326]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. The molecular defect in SCA12 is the expansion of a CAG repeat in the 5'-UTR of the gene. SIMILARITY: SwissProt: Q00005 ## Belongs to the phosphatase 2A regulatory subunit B family. & Contains 7 WD repeats. ... hide » see more »
Molecular Weight	~55 kDa

Biological Information

Immunogen

The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Gly-Glu-Glu-Asp-Ile-Asp-THr-Arg- Lys-Ile-Asn-Asn-Ser-Phe-COOH.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Protein Phosphatase 2A/B. Minimal cross reactivity against 2A/B.

Isotype

IgG

Species Reactivity

Bovine
Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_001009552

Entrez Gene Summary

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in the 5' UTR of this gene may cause a rare form of autosomal dominant spinocerebellar ataxia 12.

Gene Symbol

PPP2CB
PP2CB
PP2A-beta
EC 3.1.3.16

Purification Method

Affinity Purfied

UniProt Number

P62714

UniProt Summary

FUNCTION: SwissProt: Q00005 # The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
SIZE: 443 amino acids; 51710 Da
SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
TISSUE SPECIFICITY: Brain.
DISEASE: SwissProt: Q00005 # Defects in PPP2R2B are the cause of spinocerebellar ataxia 12 (SCA12) [MIM:604326]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. The molecular defect in SCA12 is the expansion of a CAG repeat in the 5'-UTR of the gene.
SIMILARITY: SwissProt: Q00005 ## Belongs to the phosphatase 2A regulatory subunit B family. & Contains 7 WD repeats. ... hide » see more »

Molecular Weight

~55 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on NIH/3T3 lysates. Western Blot Analysis: 1:500 dilution of this lot detected PP2A/B BETA on 10 μg of NIH/3T3 lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on NIH/3T3 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP2A/B BETA on 10 μg of NIH/3T3 lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-PP2A, B b subunit (rabbit polyclonal)

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