Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, B, Po	ICC, IHC, IH(P), WB	Ch	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R, B, Po

ICC, IHC, IH(P), WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB5541
Replaces	04-1031; 04-1062
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Glial Fibrillary Acidic Protein Antibody
Alternate Names	GFAP
Background Information	Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

Description

Catalogue Number

AB5541

Replaces

04-1031; 04-1062

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Glial Fibrillary Acidic Protein Antibody

Alternate Names

GFAP

Background Information

Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

Product Information
Format	Affinity Purified
Control	Astrocytes, astrocytomas, neural stem cells.
Presentation	Purified chicken polyclonal in buffer containing PBS containing 0.02% sodium azide.

Product Information

Format

Affinity Purified

Control

Astrocytes, astrocytomas, neural stem cells.

Presentation

Purified chicken polyclonal in buffer containing PBS containing 0.02% sodium azide.

Applications
Application	This Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.
Key Applications	Immunocytochemistry Immunohistochemistry Immunohistochemistry (Paraffin) Western Blotting
Application Notes	Immunohistochemistry(paraffin): Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain. Immunocytochemistry: 1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol. Western blot: 1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa. Immunohistochemistry: 1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde. Optimal working dilutions must be determined by the end user. STAINING PATTERN: The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.

Applications

Application

This Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.

Key Applications

Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Western Blotting

Application Notes

Immunohistochemistry(paraffin):
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.

Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.

Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.

Optimal working dilutions must be determined by the end user.

STAINING PATTERN:

The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.

Biological Information
Immunogen	Purified bovine GFAP.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Chicken
Specificity	Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
Species Reactivity	Human Mouse Rat Bovine Pig
Species Reactivity Note	Human, rat, and mouse. Expected to cross-react with bovine and porcine. Other species have not yet been tested.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_002055.2
Entrez Gene Summary	This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Gene Symbol	GFAP FLJ45472
Purification Method	ImmunoAffinity Purified
UniProt Number	P14136
UniProt Summary	FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. SIZE: 432 amino acids; 49880 Da SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1. SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments. DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family. ...
Molecular Weight	55 kDa

Biological Information

Immunogen

Purified bovine GFAP.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Chicken

Specificity

Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.

Species Reactivity

Human
Mouse
Rat
Bovine
Pig

Species Reactivity Note

Human, rat, and mouse. Expected to cross-react with bovine and porcine. Other species have not yet been tested.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_002055.2

Entrez Gene Summary

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.

Gene Symbol

GFAP
FLJ45472

Purification Method

ImmunoAffinity Purified

UniProt Number

P14136

UniProt Summary

FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family. ...

Molecular Weight

55 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	50 µL

Packaging Information

Material Size

50 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal

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