Anti-TCF-4, clone 6H5-3 (mouse monoclonal IgG2a)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	EMSA, ICC, IHC, IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

EMSA, ICC, IHC, IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-511
Replaces	04-1080
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-TCF-4 Antibody, clone 6H5-3
Alternate Names	HMG box transcription factor 4 Transcription factor 7-like 2 T-Cell factor 4, T-cell-specific, HMG box T-Cell-specific transcription factor 4 Transcription factor 7-like 2
Background Information	TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.

Description

Catalogue Number

05-511

Replaces

04-1080

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-TCF-4 Antibody, clone 6H5-3

Alternate Names

HMG box transcription factor 4
Transcription factor 7-like 2
T-Cell factor 4, T-cell-specific, HMG box
T-Cell-specific transcription factor 4
Transcription factor 7-like 2

Background Information

TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.

Product Information
Format	Purified
Control	DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
Presentation	Purified mouse monoclonal IgG2a in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

Product Information

Format

Purified

Control

DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.

Presentation

Purified mouse monoclonal IgG2a in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

Applications
Application	Use Anti-TCF-4 Antibody, clone 6H5-3 (Mouse Monoclonal Antibody) validated in EMSA, ICC, IHC, IP, WB to detect TCF-4 also known as Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2.
Key Applications	Electrophoretic Mobility Shift Assay Immunocytochemistry Immunohistochemistry Immunoprecipitation Western Blotting
Application Notes	Immunocytochemistry/Immunohistochemistry: A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003). Immunoprecipitation: A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001). Supershift Assay: A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).

Applications

Application

Use Anti-TCF-4 Antibody, clone 6H5-3 (Mouse Monoclonal Antibody) validated in EMSA, ICC, IHC, IP, WB to detect TCF-4 also known as Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2.

Key Applications

Electrophoretic Mobility Shift Assay
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blotting

Application Notes

Immunocytochemistry/Immunohistochemistry:
A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).

Immunoprecipitation:
A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001).

Supershift Assay:
A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).

Biological Information
Immunogen	Amino acids 31-331 of human TCF-4.
Clone	6H5-3
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes TCF-4
Isotype	IgG2a
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	CAB97213.1
Entrez Gene Summary	SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and vertebrates, and transcription factor-like proteins, TCF-1, -3, -4, and LEF-1. They appear to bind the minor groove of the A/T C A A A G/C-motif.
Gene Symbol	TCF4 TCF-4 TCF7L2
Purification Method	Protein G Chromatography
UniProt Number	Q9NQB0
UniProt Summary	FUNCTION: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif CCTTTGATC in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1 (By similarity). Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. SIZE: 619 amino acids; 67919 Da SUBUNIT: Interacts with TGFB1I1. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300 (By similarity). SUBCELLULAR LOCATION: Nucleus. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies (By similarity). TISSUE SPECIFICITY: Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom. DISEASE: Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC). SIMILARITY: Contains 1 HMG box DNA-binding domain.
Molecular Weight	Mr 66 kDa

Biological Information

Immunogen

Amino acids 31-331 of human TCF-4.

Clone

6H5-3

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes TCF-4

Isotype

IgG2a

Species Reactivity

Human
Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

CAB97213.1

Entrez Gene Summary

SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and vertebrates, and transcription factor-like proteins, TCF-1, -3, -4, and LEF-1. They appear to bind the minor groove of the A/T C A A A G/C-motif.

Gene Symbol

TCF4
TCF-4
TCF7L2

Purification Method

Protein G Chromatography

UniProt Number

Q9NQB0

UniProt Summary

FUNCTION: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif CCTTTGATC in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1 (By similarity). Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
SIZE: 619 amino acids; 67919 Da
SUBUNIT: Interacts with TGFB1I1. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300 (By similarity).
SUBCELLULAR LOCATION: Nucleus. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies (By similarity).
TISSUE SPECIFICITY: Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.
DISEASE: Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC).
SIMILARITY: Contains 1 HMG box DNA-binding domain.

Molecular Weight

Mr 66 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by western blot on RIPA lysates of Cos-1 cells expressing transfected TCF-4. Western Blot Analysis: 0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174).
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by western blot on RIPA lysates of Cos-1 cells expressing transfected TCF-4.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174).

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-TCF-4, clone 6H5-3 (mouse monoclonal IgG2a)

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