Anti-Caspase-3 (Pro), clone E83-103, Rabbit Monoclonal

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IF, IHC, IH(P), WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

IF, IHC, IH(P), WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB3623
Replaces	04-440; 04-1088
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Caspase 3 Antibody, active (cleaved) form
Alternate Names	Apopain Yama Protein CASP3 SCA-1 procaspase3
Background Information	Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

Description

Catalogue Number

AB3623

Replaces

04-440; 04-1088

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Caspase 3 Antibody, active (cleaved) form

Alternate Names

Apopain
Yama Protein
CASP3
SCA-1
procaspase3

Background Information

Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

Product Information
Format	Affinity Purified
Control	Tonsil and appendix tissue. Jurkat cells induced for apoptosis with recombinant soluble TRAIL and staurosporine treated mouse 3T3 cell lysates Skin/Tumor, Embryo.
Presentation	Purified rabbit polyclonal in buffer containing PBS, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Product Information

Format

Affinity Purified

Control

Tonsil and appendix tissue. Jurkat cells induced for apoptosis with recombinant soluble TRAIL and staurosporine treated mouse 3T3 cell lysates Skin/Tumor, Embryo.

Presentation

Purified rabbit polyclonal in buffer containing PBS, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Applications
Application	Anti-Caspase 3 Antibody, active (cleaved) form detects level of Caspase 3 and has been published and validated for use in Immunofluorescence (IF), Immunohistochemistry (IHC), and Western Blot (WB).
Key Applications	Immunofluorescence Immunohistochemistry Immunohistochemistry (Paraffin) Western Blotting
Application Notes	Western Blot Analysis: 1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3. Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal. Immunohistochemistry: 1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues. Immunohistochemistry(paraffin): Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine

Applications

Application

Anti-Caspase 3 Antibody, active (cleaved) form detects level of Caspase 3 and has been published and validated for use in Immunofluorescence (IF), Immunohistochemistry (IHC), and Western Blot (WB).

Key Applications

Immunofluorescence
Immunohistochemistry
Immunohistochemistry (Paraffin)
Western Blotting

Application Notes

Western Blot Analysis:
1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.

Immunohistochemistry:
1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.

Immunohistochemistry(paraffin):
Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine

Biological Information
Immunogen	Cleavage specific synthetic peptide sequence (proprietary).
Epitope	active (cleaved) form
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	AB3623 detects only the cleaved p17 fragment and does not detect the precursor form in cells undergoing apoptosis.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human, Mouse, Rat. Reactivity with other species has not been determined.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004346.3
Entrez Gene Summary	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Gene Symbol	CASP3 CPP-32 Yama CASP-3 CPP32B SCA-1 CPP32 EC 3.4.22.56 [Contains: Caspase-3 subunit p17 Caspase-3 subunit p12].
Purification Method	ImmunoAffinity Purified
UniProt Number	P42574
UniProt Summary	FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. SIZE: 277 amino acids; 31608 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
Molecular Weight	17 kDa

Biological Information

Immunogen

Cleavage specific synthetic peptide sequence (proprietary).

Epitope

active (cleaved) form

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

AB3623 detects only the cleaved p17 fragment and does not detect the precursor form in cells undergoing apoptosis.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Human, Mouse, Rat. Reactivity with other species has not been determined.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_004346.3

Entrez Gene Summary

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Gene Symbol

CASP3
CPP-32
Yama
CASP-3
CPP32B
SCA-1
CPP32
EC 3.4.22.56 [Contains: Caspase-3 subunit p17 Caspase-3 subunit p12].

Purification Method

ImmunoAffinity Purified

UniProt Number

P42574

UniProt Summary

FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
SIZE: 277 amino acids; 31608 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.

Molecular Weight

17 kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Caspase-3 (Pro), clone E83-103, Rabbit Monoclonal

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