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Anti-Apelin Receptor/APJ, clone 3C3-7 (mouse monoclonal)

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货号：MABN1846

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IH(P), WB M Purified Monoclonal Antibody
Description
Catalogue Number MABN1846
Description Anti-Apelin Receptor/APJ, clone 3C3-7 Antibody
Alternate Names
  • Apelin receptor
  • Angiotensin receptor-like 1
  • G-protein coupled receptor APJ
  • G-protein coupled receptor HG11
Background Information Apelin receptor (UniProt P35414; also known as Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11) is encoded by the APLNR (also known as AGTRL1, APJ) gene (Gene ID 187) in human. Apelin is an angiotensin converting enzyme 2 (ACE2) substrate that functions as an inotropic and cardiovascular protective peptide. Apelin exerts its physiological effects via its receptor APJ, a seven-transmembrane G protein-coupled receptor that shares significant homology with the Ang II type 1 receptor (AT1R). Apelin/APJ signaling plays an important role in a wide range of pathophysiological effects in the cardiovascular system. Apelin levels are reduced in patients with essential hypertension independent of left ventricular systolic and diastolic dysfunction. Treatment with Apelin has been shown to lower systolic blood pressure levels in hypertensive rats via an NO-dependent mechanism. APJ is a 7-transmembrane (a.a. 27-51, 67-91, 101-125, 145-166, 201-221, 245-271, 285-308) GPCR, having a short N-terminal extracellular portion (a.a. 1-26) and a long C-terminal cytomplasmic tail (a.a. 309-380).
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-Apelin Receptor, clone 3C3-7 Antibody is validated for use in Immunohistochemistry (Paraffin), Western Blotting for the detection of APJ.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application Notes Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Apelin receptor/APJ in human prostate cancer tissue, as well as normal cerebral cortex and pancreas tissue sections.
Biological Information
Immunogen Recombinant full-length human Apelin receptor/APJ.
Clone 3C3-7
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Clone 3C3-7 detects human Apelin receptor/APJ.
Isotype IgG1κ
Species Reactivity
  • Human
Species Reactivity Note Human.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APLNR
  • AGTRL1
  • APJ
Purification Method Protein G purified.
UniProt Number
Molecular Weight ~42 kDa. 42.66 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Apelin receptor/APJ in 10 µg of MCF7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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