Anti-Ras, clone RAS10 (mouse monoclonal IgG)

Replacement Information
Replacement Information	05-516 is a recommended replacement for CBL751

Replacement Information

05-516 is a recommended replacement for CBL751

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M	ELISA, FC, ICC, IHC, IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R, M

ELISA, FC, ICC, IHC, IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-516
Replaces	CBL751
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Ras Antibody, clone RAS10
Alternate Names	GTP- and GDP-binding peptide B GTPase Hras Ha-Ras1 proto-oncoprotein Ras family small GTP binding protein H-Ras Transforming protein p2 c-has/bas p21 protein c-ras-Ki-2 activated oncogene p19 H-RasIDX protein transformation gene: oncogene HAMSV v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Background Information	The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.

Description

Catalogue Number

05-516

Replaces

CBL751

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Ras Antibody, clone RAS10

Alternate Names

GTP- and GDP-binding peptide B
GTPase Hras
Ha-Ras1 proto-oncoprotein
Ras family small GTP binding protein H-Ras
Transforming protein p2
c-has/bas p21 protein
c-ras-Ki-2 activated oncogene
p19 H-RasIDX protein
transformation gene: oncogene HAMSV
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Background Information

The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.

Product Information
Format	Purified
Control	Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels
Presentation	Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Product Information

Format

Purified

Control

Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels

Presentation

Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Applications
Application	Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.
Key Applications	ELISA Flow Cytometry Immunocytochemistry Immunohistochemistry Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: 1-4 μg of this antibody has been reported to immunoprecipitate Ras. Immunocytochemistry: 2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells. Immunohistochemistry: This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.

Applications

Application

Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.

Key Applications

ELISA
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blotting

Application Notes

Immunoprecipitation:
1-4 μg of this antibody has been reported to immunoprecipitate Ras.

Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.

Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.

Biological Information
Immunogen	Purified recombinant Ras. Clone RAS10.
Clone	RAS10
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes p21H-, K- and N-Ras.
Isotype	IgG2aκ
Species Reactivity	Human Rat Mouse
Species Reactivity Note	Proven to react with human, mouse and rat. Other species unknown.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_005343
Entrez Gene Summary	Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
Gene Symbol	K-Ras Ki-Ras K-Ras2 Kras-2 p21B KRAS RASK2 HRAS HA_RAS N-RAS H-RAS NRAS NRAS1 ALPS4
Purification Method	Protein G Purified
UniProt Number	P01116
UniProt Summary	FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). SIZE: 189 amino acids; 21,656 Da SUBUNIT: Interacts with PHLPP (By similarity). SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Involvement in disease: Defects in KRAS are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Defects in KRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor. Defects in KRAS are the cause of Noonan syndrome 3 (NS3) [MIM:609942]. Noonan syndrome (NS) [MIM:163950] is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS3 inheritance is autosomal dominant. Defects in KRAS are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. KRAS mutations are involved in cancer development.
Molecular Weight	21 kDa

Biological Information

Immunogen

Purified recombinant Ras. Clone RAS10.

Clone

RAS10

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes p21H-, K- and N-Ras.

Isotype

IgG2aκ

Species Reactivity

Human
Rat
Mouse

Species Reactivity Note

Proven to react with human, mouse and rat. Other species unknown.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_005343

Entrez Gene Summary

Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]

Gene Symbol

K-Ras
Ki-Ras
K-Ras2
Kras-2
p21B
KRAS
RASK2
HRAS
HA_RAS
N-RAS
H-RAS
NRAS
NRAS1
ALPS4

Purification Method

Protein G Purified

UniProt Number

P01116

UniProt Summary

FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
SIZE: 189 amino acids; 21,656 Da
SUBUNIT: Interacts with PHLPP (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side.
Involvement in disease:
Defects in KRAS are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.

Defects in KRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.

Defects in KRAS are the cause of Noonan syndrome 3 (NS3) [MIM:609942]. Noonan syndrome (NS) [MIM:163950] is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS3 inheritance is autosomal dominant.

Defects in KRAS are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.

KRAS mutations are involved in cancer development.

Molecular Weight

21 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates. Western Blot Analysis: 0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.

Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-Ras, clone RAS10 (mouse monoclonal IgG)

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