Anti-RelB (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Rhesus Macaque	WB, IH(P)	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R, Rhesus Macaque

WB, IH(P)

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	06-1105
Replaces	04-1077
Description	Anti-RelB Antibody
Alternate Names	reticuloendotheliosis viral oncogene homolog B v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3) v-rel reticuloendotheliosis viral oncogene homolog B v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in -cells 3
Background Information	NFĸB (Nuclear Factor kappa B) represents a group of transcription factors that are structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel. The five members in mammals includes Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100). NFĸB/Rel proteins exist as homo- or heterodimer complexes. In a majority of unstimulated cells, the NFĸB transcription factors are bound to the inhibitory IĸB proteins and retained in the cytoplasm in their inactive form. RelB is a component of the NFĸB RelB-p50 complex and the RelB-p52 complex. RelB's interaction in complex seems to be transient and prevents degradation, allowing for heterodimer formation with p50 or p52.

Description

Catalogue Number

06-1105

Replaces

04-1077

Description

Anti-RelB Antibody

Alternate Names

reticuloendotheliosis viral oncogene homolog B
v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
v-rel reticuloendotheliosis viral oncogene homolog B
v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in -cells 3

Background Information

NFĸB (Nuclear Factor kappa B) represents a group of transcription factors that are structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel. The five members in mammals includes Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100). NFĸB/Rel proteins exist as homo- or heterodimer complexes. In a majority of unstimulated cells, the NFĸB transcription factors are bound to the inhibitory IĸB proteins and retained in the cytoplasm in their inactive form. RelB is a component of the NFĸB RelB-p50 complex and the RelB-p52 complex. RelB's interaction in complex seems to be transient and prevents degradation, allowing for heterodimer formation with p50 or p52.

Product Information
Format	Affinity Purified
Control	Raji cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Raji cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Use Anti-RelB Antibody (rabbit polyclonal antibody) validated in WB,IHC(P) to detect RelB also known as reticuloendotheliosis viral oncogene homolog B, v-rel avian reticuloendotheliosis viral oncogene homolog B.
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected RelB in ductal carcinoma in situ tissue.

Applications

Application

Use Anti-RelB Antibody (rabbit polyclonal antibody) validated in WB,IHC(P) to detect RelB also known as reticuloendotheliosis viral oncogene homolog B, v-rel avian reticuloendotheliosis viral oncogene homolog B.

Key Applications

Western Blotting
Immunohistochemistry (Paraffin)

Application Notes

Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected RelB in ductal carcinoma in situ tissue.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human RelB at the RHD domain.
Epitope	RHD domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes RelB at the RHD domain.
Species Reactivity	Human Mouse Rat Rhesus Macaque
Species Reactivity Note	Demonstrated to react with human, mouse, and rat. Predicted to react with rhesus macaque based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_006500
Gene Symbol	I-REL I-Rel IREL RelB
Purification Method	Affinity Purfied
UniProt Number	Q01201
UniProt Summary	FUNCTION: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. SUBUNIT STRUCTURE: Component of the NF-kappa-B RelB-p50 complex. Component of the NF-kappa-B RelB-p52 complex. Self-associates; the interaction seems to be transient and may prevent degradation allowing for heterodimer formation with p50 or p52. Interacts with NFKB1/p50, NFKB2/p52 and NFKB2/p100. Interacts with NFKBID By similarity. SUBCELLULAR LOCATION: Nucleus. INDUCTION: By mitogens. DOMAIN: Both N- and C-terminal domains are required for transcriptional activation. PTM: Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation By similarity. SEQUENCE SIMILARITIES: Contains 1 RHD (Rel-like) domain. CAUTION: Was originally (Ref.1) thought to inhibit the transcriptional activity of nuclear factor NF-kappa-B.
Molecular Weight	~58 kDa observed

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human RelB at the RHD domain.

Epitope

RHD domain

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes RelB at the RHD domain.

Species Reactivity

Human
Mouse
Rat
Rhesus Macaque

Species Reactivity Note

Demonstrated to react with human, mouse, and rat.
Predicted to react with rhesus macaque based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_006500

Gene Symbol

I-REL
I-Rel
IREL
RelB

Purification Method

Affinity Purfied

UniProt Number

Q01201

UniProt Summary

FUNCTION: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.

SUBUNIT STRUCTURE: Component of the NF-kappa-B RelB-p50 complex. Component of the NF-kappa-B RelB-p52 complex. Self-associates; the interaction seems to be transient and may prevent degradation allowing for heterodimer formation with p50 or p52. Interacts with NFKB1/p50, NFKB2/p52 and NFKB2/p100. Interacts with NFKBID By similarity.

SUBCELLULAR LOCATION: Nucleus.

INDUCTION: By mitogens.

DOMAIN: Both N- and C-terminal domains are required for transcriptional activation.

PTM: Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation By similarity.

SEQUENCE SIMILARITIES: Contains 1 RHD (Rel-like) domain.

CAUTION: Was originally (Ref.1) thought to inhibit the transcriptional activity of nuclear factor NF-kappa-B.

Molecular Weight

~58 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in Raji cell lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected RelB in 10 µg of Raji cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected RelB in 10 µg of Raji cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-RelB (rabbit polyclonal)

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