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Anti-Glycine Receptor a3 subunit (rabbit polyclonal)

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货号：AB15014

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M IHC, WB Rb Purified Polyclonal Antibody
Description
Catalogue Number AB15014
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Glycine Receptor α3 subunit Antibody
Alternate Names
  • glycine receptor alpha 3 subunit
  • glycine receptor, alpha 3
  • glycine receptor
  • alpha-3 polypeptide, ligand gated ion channel
Background Information Glycine receptor α3 subunit, also known as GLRA3, belongs to the ligand-gated ion channel family. Glycine functions as a inhibitory neurotransmitter throughout the CNS of mammals. The Glycine receptor is a neurotransmitter ligand-gated ion channel. GLRA3 is a pentamer that is composed of ligand binding alpha and structural beta subunits. When glycine binds to the receptor, chloride conductance increases which produces hyperpolarization. It is expressed in the hippocampus, olfactory bulb, and cerebellum. GLRA3 may be involved in human and murine neurological disorders.
References
Product Information
Format Purified
Control
  • Fetal human brain tissue lysate
Presentation Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-Glycine Receptor α3 subunit Antibody detects level of Glycine Receptor α3 subunit & has been published & validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Immunohistochemistry Analysis: 1:100-500 dilution from a previous lot detected Glycine Receptor α3 subunit in mouse spinal cord and mouse eye tissue.
Biological Information
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated linear peptide selected from residues surrounding the C-terminus of human Glycine Receptor α3 subunit.
Epitope C-terminus
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity This antibody recognizes Glycine Receptor α3 subunit.
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Demonstrated to react with human and mouse. Rat (92%) homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GLRA3
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: O75311 # The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
SIZE: 464 amino acids; 53800 Da
SUBUNIT: Pentamer composed of alpha and beta subunits (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely distributed throughout the central nervous system.
DOMAIN: SwissProt: O75311 The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.
SIMILARITY: Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
MISCELLANEOUS: The alpha subunit binds strychnine.
Molecular Weight Approx. 48 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C for up to 12 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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