Anti-IL-16, clone 14.1 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, NEUT, IHC, ICC, FC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, NEUT, IHC, ICC, FC

Purified

Monoclonal Antibody

Description
Catalogue Number	MABF29
Description	Anti-IL-16 Antibody, clone 14.1
Alternate Names	interleukin 16 (lymphocyte chemoattractant factor) lymphocyte chemoattractant factor pro-interleukin-16 neuronal interleukin 16 prointerleukin 16
Background Information	Originally known as lymphocyte chemoattractant factor (LCF), Interleukin-16 (IL-16) is a product of human peripheral mononuclear cells when antigen-stimulated or mitogen-stimulated. It is also produced by dendritic cells, epithelial cells, eosinophils, fibroblasts and mast cells. The pro-form of IL-16 is a 631 amino acid protein that is cleaved by caspase 3 in activated CD4+ T cells where it is then secreted and is accessible for binding CD4 receptors. IL-16 regulates and induces migratory response in CD4+ lymphocytes, eosinophils, and monocytes. It is a soluble ligand for CD4 and functions to prime responsiveness to IL-2 and IL-15 in CD4+ T-cells. Although Isoform 1’s function is thought to be as an anchor to membrane ion channels, Isoform 3 is more widely researched and characterized as component of T-cell cycle progression.

Description

Catalogue Number

MABF29

Description

Anti-IL-16 Antibody, clone 14.1

Alternate Names

interleukin 16 (lymphocyte chemoattractant factor)
lymphocyte chemoattractant factor
pro-interleukin-16
neuronal interleukin 16
prointerleukin 16

Background Information

Originally known as lymphocyte chemoattractant factor (LCF), Interleukin-16 (IL-16) is a product of human peripheral mononuclear cells when antigen-stimulated or mitogen-stimulated. It is also produced by dendritic cells, epithelial cells, eosinophils, fibroblasts and mast cells. The pro-form of IL-16 is a 631 amino acid protein that is cleaved by caspase 3 in activated CD4+ T cells where it is then secreted and is accessible for binding CD4 receptors. IL-16 regulates and induces migratory response in CD4+ lymphocytes, eosinophils, and monocytes. It is a soluble ligand for CD4 and functions to prime responsiveness to IL-2 and IL-15 in CD4+ T-cells. Although Isoform 1’s function is thought to be as an anchor to membrane ion channels, Isoform 3 is more widely researched and characterized as component of T-cell cycle progression.

Product Information
Format	Purified
Control	Human serum
Presentation	Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

Human serum

Presentation

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Detect IL-16 using this Anti-IL-16 Antibody, clone 14.1 validated for use in WB, NEUT, IH, IC, FC.
Key Applications	Western Blotting Neutralizing Immunohistochemistry Immunocytochemistry Flow Cytometry
Application Notes	Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH to detect IL-16 in naive kidney tissue (Wang, S., et al., 2008). Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC to detect IL-16 in spinal cord cells (Skundric, D., et al., 2005). Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect IL-16 in human PBMCs (McFadden, C., et al., 2007). Neutralizing Activity Assay: 1–10 µg/mL of a previous lot was used by an independent laboratory at concentrations sufficient to neutralize bioactivity of IL-16 (Morgan, R., et al., 2007).

Applications

Application

Detect IL-16 using this Anti-IL-16 Antibody, clone 14.1 validated for use in WB, NEUT, IH, IC, FC.

Key Applications

Western Blotting
Neutralizing
Immunohistochemistry
Immunocytochemistry
Flow Cytometry

Application Notes

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH to detect IL-16 in naive kidney tissue (Wang, S., et al., 2008).

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC to detect IL-16 in spinal cord cells (Skundric, D., et al., 2005).

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect IL-16 in human PBMCs (McFadden, C., et al., 2007).

Neutralizing Activity Assay: 1–10 µg/mL of a previous lot was used by an independent laboratory at concentrations sufficient to neutralize bioactivity of IL-16 (Morgan, R., et al., 2007).

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the C-terminal region of secreted human IL-16.
Epitope	Unknown
Clone	14.1
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001165599
Entrez Gene Summary	The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq].
Gene Symbol	IL-16 PRIL16 LCF IL16 NIL16 prIL-16
Purification Method	Protein G Purified
UniProt Number	Q14005
UniProt Summary	FUNCTION: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane. Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. SUBUNIT STRUCTURE: Homotetramer Probable; according (Ref.19) the formation of a homotetrameric protein complex is not required for the chemo-attractant function. Isoform 3 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GRIN2A. Isoform 3 interacts with GABPB1. Isoform 3 interacts (via PDZ 3 domain) with HDAC3. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C By similarity. Isoform 3 interacts with HTLV-1 tax. SUBCELLULAR LOCATION: Interleukin-16: Secreted Isoform 1: Cytoplasm (By similarity). Isoform 3: Cytoplasm. Nucleus TISSUE SPECIFICITY: Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation. INDUCTION: Isoform 3 is down-regulated in T-cells after TCR activation. PTM: Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. SEQUENCE SIMILARITIES: Contains 4 PDZ (DHR) domains.
Molecular Weight	~20 kDa observed. Monomers of IL-16 migrate on SDS-PAGE in the range of 17–20 kDa (Zhang, Y., et al., 1998). Active IL-16 (clone 14.1) is detected at ~17 kDa in WB (Skundric, D., et al., 2005).

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to the C-terminal region of secreted human IL-16.

Epitope

Unknown

Clone

14.1

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001165599

Entrez Gene Summary

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq].

Gene Symbol

IL-16
PRIL16
LCF
IL16
NIL16
prIL-16

Purification Method

Protein G Purified

UniProt Number

Q14005

UniProt Summary

FUNCTION: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.

Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.

Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

SUBUNIT STRUCTURE: Homotetramer Probable; according (Ref.19) the formation of a homotetrameric protein complex is not required for the chemo-attractant function. Isoform 3 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GRIN2A. Isoform 3 interacts with GABPB1. Isoform 3 interacts (via PDZ 3 domain) with HDAC3. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C By similarity. Isoform 3 interacts with HTLV-1 tax.

SUBCELLULAR LOCATION: Interleukin-16: Secreted

Isoform 1: Cytoplasm (By similarity).

Isoform 3: Cytoplasm. Nucleus

TISSUE SPECIFICITY: Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.

INDUCTION: Isoform 3 is down-regulated in T-cells after TCR activation.

PTM: Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.

SEQUENCE SIMILARITIES: Contains 4 PDZ (DHR) domains.

Molecular Weight

~20 kDa observed.
Monomers of IL-16 migrate on SDS-PAGE in the range of 17–20 kDa (Zhang, Y., et al., 1998). Active IL-16 (clone 14.1) is detected at ~17 kDa in WB (Skundric, D., et al., 2005).

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human serum. Western Blot Analysis: A 1:1,000 dilution of this antibody detected IL-16 in 10 µg of human serum.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human serum.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected IL-16 in 10 µg of human serum.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-IL-16, clone 14.1 (mouse monoclonal)

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