Anti-phospho-JNK (Thr183/Tyr185,Thr221/Tyr223)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H, R, Ca, Xn, Ch, B, Eq	WB, Enzyme Assays, IP	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, H, R, Ca, Xn, Ch, B, Eq

WB, Enzyme Assays, IP

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-175
Description	Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
Alternate Names	JNK1 alpha protein kinase JNK1 beta protein kinase JUN N-terminal kinase Stress-activated protein kinase JNK1 Stress-activated protein kinase JNK1 mitogen-activated protein kinase 8 mitogen-activated protein kinase 8 isoform JNK1 alpha1 mitogen-activated protein kinase 8 isoform JNK1 alpha1 protein kinase JNK1
Background Information	JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.

Description

Catalogue Number

07-175

Description

Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody

Alternate Names

JNK1 alpha protein kinase
JNK1 beta protein kinase
JUN N-terminal kinase
Stress-activated protein kinase JNK1
Stress-activated protein kinase JNK1
mitogen-activated protein kinase 8
mitogen-activated protein kinase 8 isoform JNK1 alpha1
mitogen-activated protein kinase 8 isoform JNK1 alpha1
protein kinase JNK1

Background Information

JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.

Product Information
Format	Affinity Purified
Control	Untreated and Lambda phosphatase treated RAW264.7 cell lysates
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Untreated and Lambda phosphatase treated RAW264.7 cell lysates

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.
Key Applications	Western Blotting Enzyme Assays Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate.

Applications

Application

Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.

Key Applications

Western Blotting
Enzyme Assays
Immunoprecipitation

Application Notes

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223.
Epitope	Phosphorylated at Thr183/Tyr185 and Thr221/Tyr223
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .
Isotype	IgG
Species Reactivity	Mouse Human Rat Canine Xenopus Chicken Bovine Horse
Species Reactivity Note	Demonstrated to react with mouse and human. Predicted to react with rat, canine, xenopus, chicken, bovine, and equine based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_620634
Entrez Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Gene Symbol	MAPK8 JNK1 JNK21B1/2 PRKM8 JNK-46 JNK SAPK1 JNK1A2
Modifications	Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	P45983
UniProt Summary	FUNCTION: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. COFACTOR: Magnesium. SIZE: 427 amino acids; 48296 Da SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity). DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... hide » see more »
Molecular Weight	~ 42 kDa observed. There are several isoforms between 40-50 kDa.

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223.

Epitope

Phosphorylated at Thr183/Tyr185 and Thr221/Tyr223

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .

Isotype

IgG

Species Reactivity

Mouse
Human
Rat
Canine
Xenopus
Chicken
Bovine
Horse

Species Reactivity Note

Demonstrated to react with mouse and human. Predicted to react with rat, canine, xenopus, chicken, bovine, and equine based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_620634

Entrez Gene Summary

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gene Symbol

MAPK8
JNK1
JNK21B1/2
PRKM8
JNK-46
JNK
SAPK1
JNK1A2

Modifications

Phosphorylation

Purification Method

Affinity Purfied

UniProt Number

P45983

UniProt Summary

FUNCTION: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

COFACTOR: Magnesium.

SIZE: 427 amino acids; 48296 Da

SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).

DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... hide » see more »

Molecular Weight

~ 42 kDa observed. There are several isoforms between 40-50 kDa.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates. Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-phospho-JNK (Thr183/Tyr185,Thr221/Tyr223)

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