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ANTI-IPLA2 200UL

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货号：07-169

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IHC Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 07-169-I
Description Anti-iPLA2 Antibody
Alternate Names
  • iPLA2
  • 85/88 kDa calcium-independent phospholipase A2 (EC:3.1.1.4)
  • CaI-PLA2
  • Group VI phospholipase A2
  • GVI PLA2
  • Intracellular membrane-associated calcium-independent phospholipase A2 beta
  • iPLA2-beta
  • Patatin-like phospholipase domain-containing protein 9
  • PNPLA9
Background Information Phospholipase A2 (PLA2) enzymes constitute the largest class of phospholipases, members of which catalyze the hydrolysis of the phospholipid sn-2 ester bond, generating a free fatty acid and a lysophospholipid. PLA2 enzymes are divided into 14 groups (I-XIV), including 20 subgroups (e.g. IA, IB, VIA-1, VIA-2, VIB, etc.), based on sequence homology. Historically, PLA2 enzymes have also been classified based on their biological characteristics into three types, secretory (sPLA2), cytosolic Ca2+-dependent (cPLA2), and cytosolic Ca2+-independent (iPLA2), when sequence data were not available. (PMID 12401193 & 12694560). Cat. No. 07-169-I, Anti-iPLA2, is a purified rabbit polyclonal raised against immunogen sequence present in the group VI SH-iPLA2 & LH-iPLA2 (85/88 kDa calcium-independent phospholipase A2) isoforms, but not found in the 53.2 kDa Ankyrin-iPLA2-1 and 46.5 kDa Ankyrin-iPLA2-2 splice variants encoded by the same PLA2G6 (PLPLA9) gene (UniProt O60733). Immunogen sequence is 100% conserved in murine (NP_058611.1) and rodent (AAC53136.1) species.
References
Product Information
Format Affinity Purified
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-iPLA2 Antibody is an antibody against iPLA2 for use inWestern Blotting and Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected iPLA2 in human tonsil tissue.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human iPLA2 near the C-terminal.
Epitope near C-terminal
Concentration Please refer to lot specific datasheet.
Host Rabbit
Species Reactivity
  • Human
Species Reactivity Note Human. Predicted to react with Mouse, Rat, Hamster based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PLA2G6
  • PLPLA9
Purification Method Affinity
UniProt Number
Molecular Weight ~70 kDa observed (Ramanadham, S., et al. (2003). Biochemistry. 42(47):13929-13940). This antibody is expected to cross-react to isoforms LH-iPLA2 and SH-iPLA2 only.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected iPLA2 in 10 µg of human testis tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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