Anti-phospho-Histone H2B (Ser14) (rabbit antiserum)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ELISA, ICC, IHC, WB	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

ELISA, ICC, IHC, WB

Serum

Polyclonal Antibody

Description
Catalogue Number	07-191
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-Histone H2B (Ser14) Antibody
Alternate Names	H2BS14P Histone H2B (phospho S14) H2B type 12 histone 3 H2bb histone H2B v
Background Information	Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression.

Description

Catalogue Number

07-191

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-phospho-Histone H2B (Ser14) Antibody

Alternate Names

H2BS14P
Histone H2B (phospho S14)
H2B type 12
histone 3
H2bb
histone H2B
v

Background Information

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression.

Product Information
Format	Serum
Control	Acid extracted HL-60 cells treated with VP16
Presentation	Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Product Information

Format

Serum

Control

Acid extracted HL-60 cells treated with VP16

Presentation

Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Applications
Application	Use Anti-phospho-Histone H2B (Ser14) Antibody (Rabbit Polyclonal Antibody) validated in ELISA, ICC, IHC, WB to detect phospho-Histone H2B (Ser14) also known as H2BS14P, Histone H2B (phospho S14).
Key Applications	ELISA Immunocytochemistry Immunohistochemistry Western Blotting

Applications

Application

Use Anti-phospho-Histone H2B (Ser14) Antibody (Rabbit Polyclonal Antibody) validated in ELISA, ICC, IHC, WB to detect phospho-Histone H2B (Ser14) also known as H2BS14P, Histone H2B (phospho S14).

Key Applications

ELISA
Immunocytochemistry
Immunohistochemistry
Western Blotting

Biological Information
Immunogen	KLH-conjugated, synthetic peptide corresponding to amino acids 6-16 of human histone H2B (C-SAPAPKKG[pS]KK).
Epitope	Ser14
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes phospho-Histone H2B (Ser14), Mr 15 kDa.
Isotype	IgG
Species Reactivity	Human
Species Reactivity Note	Human. Broad species cross-reactivity expected based on sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_778225
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
Gene Symbol	HIST3H2BB
Modifications	Phosphorylation
Purification Method	Unpurified
UniProt Number	Q8N257
UniProt Summary	FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 126 amino acids; 13908 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination. SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.
Molecular Weight	15 kDa

Biological Information

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 6-16 of human histone H2B (C-SAPAPKKG[pS]KK).

Epitope

Ser14

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes phospho-Histone H2B (Ser14), Mr 15 kDa.

Isotype

IgG

Species Reactivity

Human

Species Reactivity Note

Human. Broad species cross-reactivity expected based on sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_778225

Entrez Gene Summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.

Gene Symbol

HIST3H2BB

Modifications

Phosphorylation

Purification Method

Unpurified

UniProt Number

Q8N257

UniProt Summary

FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 126 amino acids; 13908 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.
SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.

Molecular Weight

15 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by western blot on acid extracted HL-60 cells treated with VP16. Western Blot Analysis: 1:1,000-1:10,000 dilutions of this lot detected phospho-Histone H2B in acid extracted HL-60 cells treated with 20μg/mL VP16 for 18 hours. A 1:250-1:2,000 dilution of a previous lot also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells. Note: The optimal antibody dilution of this antibody must be empirically determined. Use of this antibody at dilutions which are too low, will result in non-specific detection of Histone H2B and other unknown proteins in western blots of acid extracts from untreated cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by western blot on acid extracted HL-60 cells treated with VP16.

Western Blot Analysis:
1:1,000-1:10,000 dilutions of this lot detected phospho-Histone H2B in acid extracted HL-60 cells treated with 20μg/mL VP16 for 18 hours. A 1:250-1:2,000 dilution of a previous lot also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells.

Note:
The optimal antibody dilution of this antibody must be empirically determined. Use of this antibody at dilutions which are too low, will result in non-specific detection of Histone H2B and other unknown proteins in western blots of acid extracts from untreated cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-phospho-Histone H2B (Ser14) (rabbit antiserum)

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