Ms X Hu Desmoplakin 1 & 2, clone DP2.15

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, Ch, H, M, R	IF, WB, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

B, Ch, H, M, R

IF, WB, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	CBL173
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Desmoplakin 1& 2 Antibody, clone DP2.15

Description

Catalogue Number

CBL173

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Desmoplakin 1& 2 Antibody, clone DP2.15

Product Information
Format	Purified
Presentation	Lyophilized. Reconstitute in 1 mL distilled water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4

Product Information

Format

Purified

Presentation

Lyophilized. Reconstitute in 1 mL distilled water (final solution contains 0.09% sodium
azide, 0.5% BSA in PBS buffer, pH 7.4

Applications
Application	Anti-Desmoplakin 1& 2 Antibody, clone DP2.15 detects level of Desmoplakin 1& 2 & has been published & validated for use in IF, WB & IC.
Key Applications	Immunofluorescence Western Blotting Immunocytochemistry
Application Notes	Western blot Immunohistochemistry: 1:10; Frozen tissue Immunofluorescence Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-Desmoplakin 1& 2 Antibody, clone DP2.15 detects level of Desmoplakin 1& 2 & has been published & validated for use in IF, WB & IC.

Key Applications

Immunofluorescence
Western Blotting
Immunocytochemistry

Application Notes

Western blot
Immunohistochemistry: 1:10; Frozen tissue
Immunofluorescence

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Bovine desmoplakin 1 & 2
Clone	DP2.15
Host	Mouse
Specificity	This antibody shows distinct punctate membrane staining of desmoplakins 1 (MW 250 kDa) and 2 (MW 215 kDa) in stratified epithelia and simple epithelia, including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges.
Isotype	IgG1
Species Reactivity	Bovine Chicken Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001008844.1 NM_004415.2
Entrez Gene Summary	Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
Gene Symbol	DSP DPI Desmoplakin PPKS2 KPPS2 DP DPII
UniProt Number	P15924
UniProt Summary	FUNCTION: SwissProt: P15924 # Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. SIZE: 2871 amino acids; 331774 Da SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Note=Innermost portion of the desmosomal plaque. TISSUE SPECIFICITY: Isoform DPI is apparently an obligate constituent of all desmosomes; Isoform DPII resides predominantly in tissues and cells of stratified origin. DOMAIN: SwissProt: P15924 The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1. The C-terminal region interacts with intermediate filaments. PTM: Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide. DISEASE: "SwissProt: P15924 # Defects in DSP are the cause of keratosis palmoplantaris striata II (PPKS2) [MIM:125647]; also known as striate palmoplantar keratoderma II (SPPK2). PPKS2 is characterized by skin thickening in the palms (linear pattern) and the soles (island-like pattern) and flexor aspect of the fingers; and rarely by abnormalities of the nails, the teeth and the hair. & Defects in DSP are the cause of dilated cardiomyopathy with woolly hair and keratoderma (DCWHK) [MIM:605676]; also known as Carvajal syndrome or palmoplantar keratoderma with left ventricular cardiomyopathy and woolly hair. DCWHK is an autosomal recessive cardiocutaneous syndrome characterized by a generalized striate keratoderma particularly affecting the palmoplantar epidermis, woolly hair, and dilated left ventricular cardiomyopathy. & Defects in DSP are the cause of familial arrhythmogenic right ventricular dysplasia 8 (ARVD8) [MIM:607450]; also known as arrhythmogenic right ventricular cardiomyopathy 8 (ARVC8). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. & Defects in DSP are the cause of skin fragility-woolly hair syndrome (SFWHS) [MIM:607655]. SFWHS is an autosomal recessive genodermatosis characterized by focal and diffuse palmoplantar keratoderma, hyperkeratotic plaques on the trunk and limbs, and woolly hair with varying degrees of alopecia. & Defects in DSP are the cause of lethal acantholytic epidermolysis bullosa [MIM:609638]. Lethal acantholytic epidermolysis bullosa is characterized by severe fragility of skin and mucous membranes. The phenotype is lethal in the neonatal period because of immense transcutaneous fluid loss. Typical features include universal alopecia, neonatal teeth, and nail loss. Histopathology of the skin shows suprabasal clefting and acantholysis throughout the spinous layer, mimicking pemphigus." SIMILARITY: Belongs to the plakin or cytolinker family. & Contains 17 plectin repeats. & Contains 2 spectrin repeats.

Biological Information

Immunogen

Bovine desmoplakin 1 & 2

Clone

DP2.15

Host

Mouse

Specificity

This antibody shows distinct punctate membrane staining of desmoplakins 1 (MW 250 kDa) and 2 (MW 215 kDa) in stratified epithelia and simple epithelia, including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges.

Isotype

IgG1

Species Reactivity

Bovine
Chicken
Human
Mouse
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

Entrez Gene Summary

Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.

Gene Symbol

DSP
DPI
Desmoplakin
PPKS2
KPPS2
DP
DPII

UniProt Number

P15924

UniProt Summary

FUNCTION: SwissProt: P15924 # Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
SIZE: 2871 amino acids; 331774 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Note=Innermost portion of the desmosomal plaque.
TISSUE SPECIFICITY: Isoform DPI is apparently an obligate constituent of all desmosomes; Isoform DPII resides predominantly in tissues and cells of stratified origin.
DOMAIN: SwissProt: P15924 The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1. The C-terminal region interacts with intermediate filaments.
PTM: Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide.
DISEASE: "SwissProt: P15924 # Defects in DSP are the cause of keratosis palmoplantaris striata II (PPKS2) [MIM:125647]; also known as striate palmoplantar keratoderma II (SPPK2). PPKS2 is characterized by skin thickening in the palms (linear pattern) and the soles (island-like pattern) and flexor aspect of the fingers; and rarely by abnormalities of the nails, the teeth and the hair. & Defects in DSP are the cause of dilated cardiomyopathy with woolly hair and keratoderma (DCWHK) [MIM:605676]; also known as Carvajal syndrome or palmoplantar keratoderma with left ventricular cardiomyopathy and woolly hair. DCWHK is an autosomal recessive cardiocutaneous syndrome characterized by a generalized striate keratoderma particularly affecting the palmoplantar epidermis, woolly hair, and dilated left ventricular cardiomyopathy. & Defects in DSP are the cause of familial arrhythmogenic right ventricular dysplasia 8 (ARVD8) [MIM:607450]; also known as arrhythmogenic right ventricular cardiomyopathy 8 (ARVC8). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. & Defects in DSP are the cause of skin fragility-woolly hair syndrome (SFWHS) [MIM:607655]. SFWHS is an autosomal recessive genodermatosis characterized by focal and diffuse palmoplantar keratoderma, hyperkeratotic plaques on the trunk and limbs, and woolly hair with varying degrees of alopecia. & Defects in DSP are the cause of lethal acantholytic epidermolysis bullosa [MIM:609638]. Lethal acantholytic epidermolysis bullosa is characterized by severe fragility of skin and mucous membranes. The phenotype is lethal in the neonatal period because of immense transcutaneous fluid loss. Typical features include universal alopecia, neonatal teeth, and nail loss. Histopathology of the skin shows suprabasal clefting and acantholysis throughout the spinous layer, mimicking pemphigus."
SIMILARITY: Belongs to the plakin or cytolinker family. & Contains 17 plectin repeats. & Contains 2 spectrin repeats.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Reconstituted antibody can be stored at 2°-8°C for up to 12 months from date of receipt. Antibody can also be stored at -20°C in undiluted aliquots.

Storage and Shipping Information

Storage Conditions

Reconstituted antibody can be stored at 2°-8°C for up to 12 months from date of receipt. Antibody can also be stored at -20°C in undiluted aliquots.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Ms X Hu Desmoplakin 1 & 2, clone DP2.15

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