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Ms X Hu CD55

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货号：CBL511

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H FC, IP, WB, IHC, IH(P), FUNC M Purified Monoclonal Antibody
Description
Catalogue Number CBL511
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-CD55 Antibody, clone BRIC 216
Alternate Names
  • DAF
References
Product Information
Format Purified
Presentation The immunoglobulin was purified from cell culture supernatant by affinity chromatography using protein G and is presented in 1 M Tris-buffered saline, pH 7.5, containing no preservatives. The antibody has been sterile-filtered.
Applications
Application Anti-CD55 Antibody, clone BRIC 216 is an antibody against CD55 for use in FC, IP, WB, IH, IH(P).
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Affects Function
Application Notes Western blot: Non-reduced erythrocyte membrane prep; antigen is sialidase and trypsin resistant, pronase, chymotrypsin and AET sensitive
Flow Cytometry
Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded sections. Protease antigen retreival is recommended.
Immunoprecipitation
Inhibition studies


Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Human fibroblast cell line
Clone BRIC 216
Host Mouse
Specificity This antibody recognizes the CD55 antigen a 70 kDa glycoprotein also known as Decay Accelerating Factor (DAF). The antigen is present on erthrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. It is found specifically at the placental fetal-maternal interface.
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Gene Symbol
  • CD55
  • CR
  • TC
  • DAF
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P08174 # Also acts as the receptor for echovirus 7 and related viruses (echoviruses 13, 21, 29 and 33).
SIZE: 381 amino acids; 41400 Da
SUBUNIT: Monomer (major form) and non-disulfide-linked, covalent homodimer (minor form).
SUBCELLULAR LOCATION: Isoform 2: Cell membrane; Lipid-anchor, GPI- anchor.
TISSUE SPECIFICITY: Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
DOMAIN: SwissProt: P08174 The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3 (By similarity).
PTM: The Ser/Thr-rich domain is heavily O-glycosylated.
SIMILARITY: Belongs to the receptors of complement activation (RCA) family. & Contains 4 Sushi (CCP/SCR) domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Aliquot antibody into small volumes and store at -20°C for up to 12 months from the date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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