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Rb X PARP (214/215) Cleavage Site Specific

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货号：AB3565

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, H, M, R WB, ICC Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3565
Replaces 04-575; 04-576
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-PARP (214/215) cleavage site Antibody
Alternate Names
  • Poly [ADP-ribose] polymerase 1
  • PARP-1
  • NAD(+) ADP-ribosyltransferase 1
  • ADPRT 1
  • Poly[ADP-ribose] synthase 1
  • ARTD1
Background Information Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.
References
Product Information
Format Affinity Purified
Control
  • Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).
Presentation Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide
Applications
Application Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.
Biological Information
Immunogen Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.
Host Rabbit
Specificity The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Species Reactivity Note Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Gene Symbol
  • PARP1
  • poly(ADP-ribosyl)transferase
  • PARP-1
  • PARP
  • NAD
  • pADPRT-1
  • ADPRT1
  • Poly
  • ADPRT
  • PPOL
  • EC 2.4.2.30
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
SIZE: 1014 amino acids; 113084 Da
SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.
SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & Contains 1 PARP alpha-helical domain. & Contains 1 PARP catalytic domain. & Contains 2 PARP-type zinc fingers.
MISCELLANEOUS: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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