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Qiagen 凯杰 Biosharp Omega

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货号：MAB3918

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R, H WB, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number MABN1188
Replaces MAB318
Description Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2
Alternate Names
  • TH
  • Tyrosine 3-monooxygenase
  • Tyrosine 3-hydroxylase
  • Tyrosine Hydroxylase
Background Information Tyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P04177; also known as TH, Tyrosine 3-hydroxylase) is encoded by the Th gene (Gene ID 25085) in rat species. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines, dopamine and norepinephrine, from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six human TH isoforms exist as a result of alternative splicing (UniProt P07101-1 through P07101-6).
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG2bλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tyrosine Hydroxylase.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected tyrosine hydroxylase in rat brain tissue sections.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to a C-terminal region sequence of rat tyrosine hydroxylase.
Epitope C-terminal region.
Clone 10F8.2
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Clone 10F8.2 targets a C-terminal epitope present in rat and mouse TH sequence (UniProt P04177 & P24529), as well as in all six human TH spliced isoforms (UniProt P07101).
Isotype IgG2bλ
Species Reactivity
  • Rat
  • Human
Species Reactivity Note Human, Rat. Predicted to react with Mouse based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Th
Purification Method Protein G purified.
UniProt Number
Molecular Weight ~50-60 kDa observed. 55.83 kDa (rat), 55.86 kDa (mouse), 58.03/55.48/58.47/55.92/45.21/44.77 kDa (human isoform 1/2/3/4/5/6) calculated (Met1 removed). The multiplet banding pattern observed in rat PC12 lysate most likely represent differentially phosphorylated TH species (Gozal, E., et al. (2005). J. Appl. Physiol. 99(2):642-649).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected tyrosine hydroxylase in 10 µg of PC12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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