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Anti-BAF155/170, clone DXD12

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货号：04-082

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB M Purified Monoclonal Antibody
Description
Catalogue Number 04-082
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-BAF155/170 Antibody, clone DXD12
References
Product Information
Format Purified
Presentation 100 μg of Protein A purified mouse monoclonal IgG in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
Applications
Application This Anti-BAF155/170 Antibody, clone DXD12 is validated for use in WB for the detection of BAF155/170.
Key Applications
  • Western Blotting
Biological Information
Immunogen Synthetic peptide corresponding to residues 591-608 of human BAF155.
Clone clone DXD12
Host Mouse
Specificity BAF155/170
Isotype IgG
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Gene Symbol
  • SMARCC1
  • CRACC1
  • SRG3
  • BAF155
  • SWI3
  • Rsc8
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q92922 # Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex.
SIZE: 1105 amino acids; 122753 Da
SUBUNIT: Component of five multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I) and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4 or SMARCA2), and at least SMARCE1, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCC2 and SMARCB1. Other subunits specific to each of the complexes may also be present. May also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. The minimal complex composed of SMARCC1 and SMARCA4 seems to be able to associate with cyclin such as CCNE1 or transcription factors such as KLF1 or GATA1. Interacts with NR3C1 and SMARD1.
SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
PTM: Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
SIMILARITY: SwissProt: Q92922 ## Belongs to the SMARCC family. & Contains 1 SANT domain. & Contains 1 SWIRM domain.
Molecular Weight 155/170 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Immunoblot Analysis: A 1:1000 dilution of this antibody detected BAF155/170 in HeLa nuclear extracts.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C from date of shipment
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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