Anti-Desert hedgehog protein (DHH), clone 19D7.2 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, H	WB, IHC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

R, H

WB, IHC

Purified

Monoclonal Antibody

Description
Catalogue Number	04-967
Description	Anti-Desert hedgehog protein (DHH) Antibody, clone 19D7.2
Alternate Names	desert hedgehog homolog (Drosophila) desert hedgehog (Drosophila) homolog desert hedgehog
Background Information	Desert Hedgehog (DHH) is one of three signaling molecules belonging to the hedgehog family, a group of intracellular signaling molecules characterized for their regulatory ability in developmental tissue patterning. DHH is an essential component to the integrity of peripheral nerve function and structure. There is also strong support that it is critical to normal, testicular development, and the survival of spermatocytes. DHH cleaves into two chains; the DHH N-product which is localized at the cell surface, and the DHH C-product which diffuses from the cell. Defects in expression are though to cause complete pure gonadal dysgenesis 46,XY type (GDXYM), and partial gonadal dysgenesis with minifascicular neuropathy 46, XY (PGD).

Description

Catalogue Number

04-967

Description

Anti-Desert hedgehog protein (DHH) Antibody, clone 19D7.2

Alternate Names

desert hedgehog homolog (Drosophila)
desert hedgehog (Drosophila) homolog
desert hedgehog

Background Information

Desert Hedgehog (DHH) is one of three signaling molecules belonging to the hedgehog family, a group of intracellular signaling molecules characterized for their regulatory ability in developmental tissue patterning. DHH is an essential component to the integrity of peripheral nerve function and structure. There is also strong support that it is critical to normal, testicular development, and the survival of spermatocytes. DHH cleaves into two chains; the DHH N-product which is localized at the cell surface, and the DHH C-product which diffuses from the cell. Defects in expression are though to cause complete pure gonadal dysgenesis 46,XY type (GDXYM), and partial gonadal dysgenesis with minifascicular neuropathy 46, XY (PGD).

Product Information
Format	Purified
Control	Rat spinal cord tissue lysate
Presentation	Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Product Information

Format

Purified

Control

Rat spinal cord tissue lysate

Presentation

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Applications
Application	Detect Desert hedgehog protein (DHH) using this Anti-Desert hedgehog protein (DHH) Antibody, clone 19D7.2 validated for use in WB, IH.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: 1:600 dilution from a previous lot detected Desert hedgehog protein in malignant teratoma tissue.

Applications

Application

Detect Desert hedgehog protein (DHH) using this Anti-Desert hedgehog protein (DHH) Antibody, clone 19D7.2 validated for use in WB, IH.

Key Applications

Western Blotting
Immunohistochemistry

Application Notes

Immunohistochemistry Analysis: 1:600 dilution from a previous lot detected Desert hedgehog protein in malignant teratoma tissue.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Desert hedgehog protein.
Epitope	Unknown
Clone	19D7.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgMκ
Species Reactivity	Rat Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_066382
Entrez Gene Summary	This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq].
Gene Symbol	DHH HHG-3
Purification Method	Ion Exchange
UniProt Number	O43323
UniProt Summary	FUNCTION: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development. SUBCELLULAR LOCATION: Desert hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side By similarity. Note: The N-terminal peptide remains associated with the cell surface (by similarity). Desert hedgehog protein C-product: Secreted › extracellular space (by similarity). Note: The C-terminal peptide diffuses from the cell (by similarity). PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (by similarity). INVOLVEMENT IN DISEASE: Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) [MIM:607080]. PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity. Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM) [MIM:233420]; also known as male-limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks. SEQUENCE SIMILARITIES: Belongs to the hedgehog family.
Molecular Weight	~ 44 kDa observed

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human Desert hedgehog protein.

Epitope

Unknown

Clone

19D7.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Isotype

IgMκ

Species Reactivity

Rat
Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_066382

Entrez Gene Summary

This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq].

Gene Symbol

DHH
HHG-3

Purification Method

Ion Exchange

UniProt Number

O43323

UniProt Summary

FUNCTION: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

SUBCELLULAR LOCATION: Desert hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side By similarity. Note: The N-terminal peptide remains associated with the cell surface (by similarity).

Desert hedgehog protein C-product: Secreted › extracellular space (by similarity). Note: The C-terminal peptide diffuses from the cell (by similarity).

PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (by similarity).

INVOLVEMENT IN DISEASE: Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) [MIM:607080]. PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity.

Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM) [MIM:233420]; also known as male-limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks.

SEQUENCE SIMILARITIES: Belongs to the hedgehog family.

Molecular Weight

~ 44 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in rat spinal cord tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Desert hedgehog protein on 10 µg of rat spinal cord tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in rat spinal cord tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Desert hedgehog protein on 10 µg of rat spinal cord tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Desert hedgehog protein (DHH), clone 19D7.2 (mouse monoclonal)

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