Anti-phospho-Raptor (Ser877) (rabbit polyclonal)

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Anti-phospho-Raptor (Ser877) (rabbit polyclonal)

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货号：09-107

品牌：Millipore 密理博

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Pm, Mk	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	09-107
Description	Anti-phospho-Raptor (Ser877) Antibody
Alternate Names	P150 target of rapamycin (TOR)-scaffold protein p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats regulatory associated protein of mTOR
Background Information	Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates

References

Product Information
Format	Affinity Purified
Control	Jurkat cell lysate
Presentation	Purified rabbit serum in PBS Other with 50% Glycerol.

Applications
Application	This Anti-phospho-Raptor (Ser877) Antibody is validated for use in WB for the detection of phospho-Raptor (Ser877).
Key Applications	Western Blotting
Application Notes	Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Peterson, T., et al. (2009). Cell. 137:873–886.)

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Raptor phosphorylated at and around Ser877.
Epitope	Ser877
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes Raptor at Ser877.
Species Reactivity	Human Primate Monkey
Species Reactivity Note	Proven to react with human. Predicted to react with primate and monkey based on 100% sequence homology. Mouse and rat (90%).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_065812
Entrez Gene Summary	This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol	raptor Raptor
Modifications	Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	Q8N122
UniProt Summary	FUNCTION: Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions. SIZE: 1335 amino acids; 149038 Da SUBUNIT: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non- phosphorylated form of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states with different stability. TISSUE SPECIFICITY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. SIMILARITY: Contains 7 WD repeats. MISCELLANEOUS: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient availability, and its potency for dissociation is increased under nutrient-rich conditions. This action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its intrinsic catalytic activity.
Molecular Weight	~ 150 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in Jurkat cell lysate. Western Blot Analysis: 2 µg/mL of this antibody detected Raptor on 10 µg of Jurkat cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.