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Anti-mouse CD44 (H-CAM), clone KM201 (rat monoclonal)

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货号：MABT78

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M FC R Purified Monoclonal Antibody
Description
Catalogue Number MABT78
Replaces CBL1308
Description Anti-mouse CD44 (H-CAM) Antibody, clone KM201
Alternate Names
  • CD44 antigen (homing function and Indian blood group system)
  • CDw44 antigen
  • HCAM
  • H-CAM
  • HUTCH-1
  • Phagocytic glycoprotein I
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • Hyaluronate receptor
  • Epican
  • antigen gp90 homing receptor
  • chondroitin sulfate proteoglycan 8
  • hematopoietic cell E- and L-selectin ligand
Background Information CD44, also known as H-CAM is a distinct adhesion molecule that, with its 13 known isoforms, comprises a family of transmembrane glycoproteins involved in a wide variety of significant biological processes. Members belonging to this family are involved in apoptosis, migration, metastasis, tumor progression, lymphocyte homing, and the attachment of hematopoietic cells to stromal cells. CD44 is the primary cell surface receptor for hyaluronic acid (HA), and is also a notable target for WNT signaling within the intestinal mucosa. Recent studies have used CD44 marker as a means to isolate different cancer stem cells (CSC) such as; colorectal CSC, pancreatic CSC, and prostate CSC.
References
Product Information
Format Purified
Control
  • Mouse bone marrow cells
Presentation Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.
Applications
Application Detect mouse CD44 (H-CAM) using this Anti-mouse CD44 (H-CAM) Antibody, clone KM201 validated for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
Immunogen Mouse bone marrow-derived stromal cells
Epitope Near the hyaluronate binding domain
Clone KM201
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rat
Specificity The antibody recognizes CD44 near the hyaluronate binding domain.
Isotype IgG1κ
Species Reactivity
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD44
  • CD44R
  • CDW44
  • CDw44
  • CSPG8
  • ECMR-III
  • Epican
  • HCELL
  • HUTCH-I
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU3
  • MIC4
  • MUTCH-I
  • PGP-1
  • PGP-I
  • Pgp1
Purification Method Protein G
UniProt Number
UniProt Summary FUNCTION: Main cell surface receptor for hyaluronate. Adhesion to mucosal high endothelial venule and to types I and VI collagen. Probably involved in matrix adhesion, lymphocyte activation and lymph node homing.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

PTM: N-glycosylated By similarity.

O-glycosylated; contains chondroitin sulfate glycans which can be more or less sulfated (By similarity).

Phosphorylated; activation of PKC results in the dephosphorylation of Ser-742 (constitutive phosphorylation site), and the phosphorylation of Ser-708 (By similarity).

POLYMORPHISM: Two allelic forms of this glycoprotein, PGP-1.1 and PGP-1.2, have been reported. The expressed product is PGP-1.1 (Ly-24.1).

SEQUENCE SIMILARITIES: Contains 1 Link domain.
Molecular Weight 86 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Flow Cytometry in mouse bone marrow cells.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CD44 in mouse bone marrow cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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