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Qiagen 凯杰 Biosharp Omega

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Anti-Rabbit Light Chain Specific Biotin-SP-Conjugated (mouse monoclonal)

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号:MAB201B

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Rb ELISA, FC, WB, IHC M Biotin Monoclonal Antibody
Description
Catalogue Number MAB201B
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Mouse Anti-Rabbit light chain Antibody, biotin conjugate
Background Information Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
References
Product Information
Format Biotin
Presentation Lyophilized from 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer (IgG-Free, Protease-Free)
Applications
Application Mouse anti-Rabbit light chain Antibody, biotin conjugate is an antibody against Rabbit light chain for use in ELISA, FC, WB, IH.
Key Applications
  • ELISA
  • Flow Cytometry
  • Western Blotting
  • Immunohistochemistry
Application Notes Western Blotting: 1:20,000 - 1:400,000
Immunohistochemistry: 1:500 - 1:5,000 on tissue sections
Flow Cytometry: 1:200 - 1:1,000

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Prepared from rabbit IgG light chain.
Epitope Kappa light chain
Host Mouse
Specificity The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rabbit IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep immunoglobulins.
Isotype IgG1κ
Species Reactivity
  • Rabbit
Species Reactivity Note Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.
Antibody Type Monoclonal Antibody
Purification Method Ascites
Molecular Weight 25 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1.1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.
Packaging Information
Material Size 0.5 mL
Transport Information
Supplemental Information
Specifications

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