Anti-gp91-phox (rabbit polyclonal IgG)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Purified

Polyclonal Antibody

Description
Catalogue Number	07-024
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-gp91-phox Antibody
Alternate Names	Cytochrome b(558) subunit beta Cytochrome b558 subunit beta Heme-binding membrane glycoprotein gp91phox NADPH oxidase Neutrophil cytochrome b 91 kDa polypeptide Superoxide-generating NADPH oxidase heavy chain subunit chronic granulomatous disease cytochrome b-245 beta polypeptide cytochrome b-245, beta polypeptide p22 phagocyte B-cytochrome
Background Information	Cytochrome b-245, beta polypeptide (chronic granulomatous disease), also known as CYBB and P91-PHOX, is a human gene encoding a glycoprotein. Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.

Description

Catalogue Number

07-024

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-gp91-phox Antibody

Alternate Names

Cytochrome b(558) subunit beta
Cytochrome b558 subunit beta
Heme-binding membrane glycoprotein gp91phox
NADPH oxidase
Neutrophil cytochrome b 91 kDa polypeptide
Superoxide-generating NADPH oxidase heavy chain subunit
chronic granulomatous disease
cytochrome b-245 beta polypeptide
cytochrome b-245, beta polypeptide
p22 phagocyte B-cytochrome

Background Information

Cytochrome b-245, beta polypeptide (chronic granulomatous disease), also known as CYBB and P91-PHOX, is a human gene encoding a glycoprotein.
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.

Product Information
Format	Purified
Control	Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate.
Presentation	Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

Product Information

Format

Purified

Control

Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate.

Presentation

Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

Applications
Application	Anti-gp91-phox Antibody is an antibody against gp91-phox for use in WB.
Key Applications	Western Blotting
Application Notes	Immunoprecipitation: Not recommended.

Applications

Application

Anti-gp91-phox Antibody is an antibody against gp91-phox for use in WB.

Key Applications

Western Blotting

Application Notes

Immunoprecipitation:
Not recommended.

Biological Information
Immunogen	KLH-conjugated, synthetic peptide corresponding to amino acids 548-560 of human gp91-phox (KQSISNSESGPRG). The immunizing sequence is identical in mouse, and it shares 11/13 sequence homology in bovine and 10/13 in porcine.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Detects gp91-phox, Mr 75 kDa. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr 75 kDa, or appear as a smear between 75-91 kDa.
Isotype	IgG
Species Reactivity	Human
Species Reactivity Note	Human. Predicted to cross react with mouse.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000397
Entrez Gene Summary	Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Gene Symbol	CGD CGD91-phox EC 1.-.-.- GP91-1 GP91-PHOX GP91PHOX NOX2 OTTHUMP00000031915 gp91-1 gp91-phox p91-PHOX
Purification Method	Protein A Purfied
UniProt Number	P04839
UniProt Summary	FUNCTION: SwissProt: P04839 # Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. COFACTOR: FAD (Probable). SIZE: 570 amino acids; 65336 Da SUBUNIT: Composed of a heavy chain (beta) and a light chain (alpha). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. PTM: Glycosylated. DISEASE: SwissProt: P04839 # Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD) [MIM:306400]. X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections. Patients with CYBB dysfunction are generally classified into three groups, namely X91(0) (no expression of the protein); X91(-) (reduced expression) and X91(+) (normal expression). SIMILARITY: SwissProt: P04839 ## Contains 1 ferric oxidoreductase domain.
Molecular Weight	Varies. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr ~75 kDa, or appear as a smear between 75-91 kDa.

Biological Information

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 548-560 of human gp91-phox (KQSISNSESGPRG). The immunizing sequence is identical in mouse, and it shares 11/13 sequence homology in bovine and 10/13 in porcine.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Detects gp91-phox, Mr 75 kDa. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr 75 kDa, or appear as a smear between 75-91 kDa.

Isotype

IgG

Species Reactivity

Human

Species Reactivity Note

Human. Predicted to cross react with mouse.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_000397

Entrez Gene Summary

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.

Gene Symbol

CGD
CGD91-phox
EC 1.-.-.-
GP91-1
GP91-PHOX
GP91PHOX
NOX2
OTTHUMP00000031915
gp91-1
gp91-phox
p91-PHOX

Purification Method

Protein A Purfied

UniProt Number

P04839

UniProt Summary

FUNCTION: SwissProt: P04839 # Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
COFACTOR: FAD (Probable).
SIZE: 570 amino acids; 65336 Da
SUBUNIT: Composed of a heavy chain (beta) and a light chain (alpha).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
PTM: Glycosylated.
DISEASE: SwissProt: P04839 # Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD) [MIM:306400]. X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections. Patients with CYBB dysfunction are generally classified into three groups, namely X91(0) (no expression of the protein); X91(-) (reduced expression) and X91(+) (normal expression).
SIMILARITY: SwissProt: P04839 ## Contains 1 ferric oxidoreductase domain.

Molecular Weight

Varies. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr ~75 kDa, or appear as a smear between 75-91 kDa.

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on human neutrophil membranes. Western Blot Analysis: 1-4 µg/mL of this lot detected gp91-phox in a HL60 membrane and RIPA cell lysate. A previous lot detected gp91-phox in human neutrophil membranes.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on human neutrophil membranes.

Western Blot Analysis:
1-4 µg/mL of this lot detected gp91-phox in a HL60 membrane and RIPA cell lysate. A previous lot detected gp91-phox in human neutrophil membranes.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-gp91-phox (rabbit polyclonal IgG)

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