Anti-Tip60 (rabbit polyclonal IgG)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

Purified

Polyclonal Antibody

Description
Catalogue Number	07-038
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Tip60 Antibody
Alternate Names	60 kDa Tat interactive protein HIV-1 Tat interactive protein HIV-1 Tat interactive protein 60kDa K(lysine) acetyltransferase 5 K-acetyltransferase Tat interacting protein, 60kDa cPLA(2)-interacting protein cPLA2 interacting protein Histone acetyltransferase KAT5 KAT5
Background Information	Histone acetyltransferase HTATIP (Tip60). Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Direct binding of HTATIP to the TAT protein of the human immunodeficiency virus (HIV) might be an important feature for efficient TAT transactivation of HIV gene expression.

Description

Catalogue Number

07-038

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Tip60 Antibody

Alternate Names

60 kDa Tat interactive protein
HIV-1 Tat interactive protein
HIV-1 Tat interactive protein
60kDa
K(lysine) acetyltransferase 5
K-acetyltransferase
Tat interacting protein, 60kDa
cPLA(2)-interacting protein
cPLA2 interacting protein
Histone acetyltransferase KAT5
KAT5

Background Information

Histone acetyltransferase HTATIP (Tip60). Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Direct binding of HTATIP to the TAT protein of the human immunodeficiency virus (HIV) might be an important feature for efficient TAT transactivation of HIV gene expression.

Product Information
Format	Purified
Control	Jurkat cell lysate and RIPA lysates
Presentation	Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.

Product Information

Format

Purified

Control

Jurkat cell lysate and RIPA lysates

Presentation

Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.

Applications
Application	Use Anti-Tip60 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Tip60 also known as HIV-1 Tat interactive protein, K(lysine) acetyltransferase 5, K-acetyltransferase.
Key Applications	Western Blotting

Applications

Application

Use Anti-Tip60 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Tip60 also known as HIV-1 Tat interactive protein, K(lysine) acetyltransferase 5, K-acetyltransferase.

Key Applications

Western Blotting

Biological Information
Immunogen	KLH-conjugated, synthetic peptide corresponding to amino acids 494-513 (RIDSKCLHF-TPKDWSKRGKW) of human Tip60.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Tip60, Mr 60 kDa. Additional proteins were detected at 66 kDa and 80 kDa in some human and mouse cell lines.
Isotype	IgG
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_182710
Entrez Gene Summary	The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants.
Gene Symbol	EC 2.3.1.48 EC 2.3.1.- ESA1 HTATIP HTATIP1 PLIP TIP TIP60 Tip60 cPLA2
Purification Method	Protein A Purfied
UniProt Number	Q92993
UniProt Summary	FUNCTION: SwissProt: Q92993 # Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to HTATIP polyubiquitination and targets it to degradation. SIZE: 513 amino acids; 58582 Da SUBUNIT: Interacts with the cytoplasmic tail of APP (By similarity). Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Interacts with ATM. Interacts with PHF17. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Nucleus. Cytoplasm, perinuclear region. Note=Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region. PTM: Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity. & Ubiquitinated by MDM2, leading to its proteasome-dependent degradation. SIMILARITY: SwissProt: Q92993 ## Belongs to the MYST (SAS/MOZ) family.
Molecular Weight	Approx. 60 kDa

Biological Information

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 494-513 (RIDSKCLHF-TPKDWSKRGKW) of human Tip60.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Tip60, Mr 60 kDa. Additional proteins were detected at 66 kDa and 80 kDa in some human and mouse cell lines.

Isotype

IgG

Species Reactivity

Human
Mouse

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_182710

Entrez Gene Summary

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants.

Gene Symbol

EC 2.3.1.48
EC 2.3.1.-
ESA1
HTATIP
HTATIP1
PLIP
TIP
TIP60
Tip60
cPLA2

Purification Method

Protein A Purfied

UniProt Number

Q92993

UniProt Summary

FUNCTION: SwissProt: Q92993 # Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to HTATIP polyubiquitination and targets it to degradation.
SIZE: 513 amino acids; 58582 Da
SUBUNIT: Interacts with the cytoplasmic tail of APP (By similarity). Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Interacts with ATM. Interacts with PHF17. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm, perinuclear region. Note=Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
PTM: Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity. & Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
SIMILARITY: SwissProt: Q92993 ## Belongs to the MYST (SAS/MOZ) family.

Molecular Weight

Approx. 60 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by western blot on RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA. Western Blot Analysis: 0.5-2 μg/mL of this lot detected Tip60 in RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA.
Sales Restrictions	Due to license agreement restrictions, this product cannot be purchased for resale.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by western blot on RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected Tip60 in RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA.

Sales Restrictions

Due to license agreement restrictions, this product cannot be purchased for resale.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Tip60 (rabbit polyclonal IgG)

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