Anti-GST M1 (mu) (rabbit polyclonal IgG)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ELISA	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, ELISA

Purified

Polyclonal Antibody

Description
Catalogue Number	07-2054
Description	Anti-MBD1 Antibody, CT
Alternate Names	CXXC-type zinc finger protein 3 Methyl-CpG-binding protein MBD1 Protein containing methyl-CpG-binding domain 1 methyl-CpG binding domain protein 1 methyl-CpG binding domain protein 1 isoform PCM1 the regulator of fibroblast growth factor 2 (FGF-2) transcription
Background Information	DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, regulates gene expression and is critical for proper development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has been shown to play a role in chromatin modification through interaction with the histone H3K9 methyltransferase SETDB1. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that each contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity.

Description

Catalogue Number

07-2054

Description

Anti-MBD1 Antibody, CT

Alternate Names

CXXC-type zinc finger protein 3
Methyl-CpG-binding protein MBD1
Protein containing methyl-CpG-binding domain 1
methyl-CpG binding domain protein 1
methyl-CpG binding domain protein 1 isoform PCM1
the regulator of fibroblast growth factor 2 (FGF-2)
transcription

Background Information

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, regulates gene expression and is critical for proper development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has been shown to play a role in chromatin modification through interaction with the histone H3K9 methyltransferase SETDB1. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that each contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity.

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.09% NaN3.

Product Information

Format

Purified

Control

HeLa cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.09% NaN3.

Applications
Application	Anti-MBD1 Antibody, C-terminus is a Rabbit Polyclonal Antibody for detection of MBD1 also known as CXXC-type zinc finger protein 3 & has been validated in WB, ELISA.
Key Applications	Western Blotting ELISA
Application Notes	ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).

Applications

Application

Anti-MBD1 Antibody, C-terminus is a Rabbit Polyclonal Antibody for detection of MBD1 also known as CXXC-type zinc finger protein 3 & has been validated in WB, ELISA.

Key Applications

Western Blotting
ELISA

Application Notes

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).

Biological Information
Immunogen	The immunogen was a KLH-conjugated synthetic peptide corresponding the C-terminal region of human MBD1.
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes human MBD1.
Species Reactivity	Human
Species Reactivity Note	Human. Not tested in other species.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_079103
Entrez Gene Summary	This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Gene Symbol	CXXC3 OTTHUMP00000163504 OTTHUMP00000163506 OTTHUMP00000163507 PCM1 RFT
Purification Method	Protein G Chromatography
UniProt Number	Q9UIS9
UniProt Summary	FUNCTION:Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. Ref.1 Ref.3 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.21 SUBUNIT STRUCTURE: Binds OASL, AFT7IP and AFT7IP2. Binds CHAF1A and the SUV39H1-CBX5 complex via the MBD domain. Binds MGP via the TRD domain. May be part of the MeCP1 complex. During DNA replication, it recruits SETDB1 to form a S phase-specific complex that facilitates methylation of H3 'Lys-9' during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1. SUBCELLULAR LOCATION: Nucleus speckle. Note= Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. Ref.1 Ref.3 Ref.8 Ref.9 Ref.12 Ref.21 TISSUE SPECIFICITY: Widely expressed. Ref.1 INDUCTION: Up-regulated by interferon. Ref.4 DOMAIN:The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions. The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides. The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions. PTM: Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP. Ref.16 Ref.17 Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.16 Ref.15 Ref.18 Ref.19 SEQUENCE SIMILARITY: Contains 3 CXXC-type zinc fingers. Contains 1 MBD (methyl-CpG-binding) domain.
Molecular Weight	~61 kDa

Biological Information

Immunogen

The immunogen was a KLH-conjugated synthetic peptide corresponding the C-terminal region of human MBD1.

Epitope

C-terminus

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes human MBD1.

Species Reactivity

Human

Species Reactivity Note

Human. Not tested in other species.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_079103

Entrez Gene Summary

This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Gene Symbol

CXXC3
OTTHUMP00000163504
OTTHUMP00000163506
OTTHUMP00000163507
PCM1
RFT

Purification Method

Protein G Chromatography

UniProt Number

Q9UIS9

UniProt Summary

FUNCTION:Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. Ref.1 Ref.3 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.21

SUBUNIT STRUCTURE: Binds OASL, AFT7IP and AFT7IP2. Binds CHAF1A and the SUV39H1-CBX5 complex via the MBD domain. Binds MGP via the TRD domain. May be part of the MeCP1 complex. During DNA replication, it recruits SETDB1 to form a S phase-specific complex that facilitates methylation of H3 'Lys-9' during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1.

SUBCELLULAR LOCATION: Nucleus speckle. Note= Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. Ref.1 Ref.3 Ref.8 Ref.9 Ref.12 Ref.21

TISSUE SPECIFICITY: Widely expressed. Ref.1

INDUCTION: Up-regulated by interferon. Ref.4

DOMAIN:The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.

The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.

The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.

PTM: Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP. Ref.16 Ref.17

Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.16 Ref.15 Ref.18 Ref.19

SEQUENCE SIMILARITY: Contains 3 CXXC-type zinc fingers.

Contains 1 MBD (methyl-CpG-binding) domain.

Molecular Weight

~61 kDa

Product Usage Statements
Quality Assurance	Western Blot Analysis: This antibody recognizes MBD1 on 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Western Blot Analysis:
This antibody recognizes MBD1 on 10 µg of HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-GST M1 (mu) (rabbit polyclonal IgG)

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