Anti-BrdU, clone IIB5 (Mouse Monoclonal) - 炼石商城

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Anti-BrdU, clone IIB5 (Mouse Monoclonal)

市场价：: 0.00

促销价：: ¥0.00

货号：MAB3222

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
A	FC, ICC, IH(P)	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB3222
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-BrdU Antibody, clone IIB5
Alternate Names	BrdU
Background Information	The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

References

Product Information
Format	Purified
Control	After incorporation of BrdU, all DNA containing species
Presentation	Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.

Applications
Application	Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).
Key Applications	Flow Cytometry Immunocytochemistry Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See: http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols. Flow Cytometry: 1:100-1:200; acid methanol treatment required. Optimal working dilutions must be determined by the end user. In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)

Biological Information
Immunogen	A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.
Clone	IIB5
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.
Isotype	IgG1
Species Reactivity	All
Antibody Type	Monoclonal Antibody
Purification Method	Protein A Purfied

Physicochemical Information

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Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	100 µg

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