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Anti-Vesicular Glutamate Transporter 1 (Mouse Monoclonal)

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货号：MAB5502

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R, M IHC, WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB5502
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Vesicular Glutamate Transporter 1 Antibody
Alternate Names
  • Solute carrier family 17 member 7
  • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
  • vGluT1
Background Information VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate. Vesicular glutamate transporters pack the neurotransmitter into synaptic vesicles so that they can be released into the synapse. VGLUTs are dependent on a proton gradient that they create by hydrolysing adenosine triphosphate (ATP). VGLUTs have only between one hundredth and one thousandth the affinity for glutamate that EAATs have.
References
Product Information
Format Purified
Control
  • Brain
  • VGLUT1 Control Peptide AG208
Presentation Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.1% sodium azide.
Applications
Application This Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Cat. No. MAB5502, is validated for use in IH, WB for the detection of VGluT1.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Immunohistochemistry:
A previous lot of this antibody was tested on IH.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Recombinant protein from rat VGLUT1.
Clone 3C10.2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Reacts with VGLUT1 (Vesicular Glutamate Transporter 1).
Isotype IgG1
Species Reactivity
  • Rat
  • Mouse
Species Reactivity Note Expected to react with rat based on sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary a Na(+)-dependent inorganic phosphate cotransporter.
Gene Symbol
  • VGLUT1
  • BNPI
  • Slc17a7
  • Bnpi
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
SIZE: 560 amino acids; 61665 Da [MW of unprocessed precursor]
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein (Potential). Cell junction, synapse, synaptosome.
TISSUE SPECIFICITY: Expressed in the cerebellum, cerberal cortex and hippocampus. Isoform 2 is expressed specifically in retina.
SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
Molecular Weight 60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Vesicular Glutamate Transporter 1 on 10 μg of Mouse Brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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