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Anti-phospho-Src (Tyr418) (rabbit polyclonal)

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货号：07-909

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Ch, H, M ICC, IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 07-909
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-Src (Tyr418) Antibody
Alternate Names
  • c-Src
  • p60-Src Src kinase
References
Product Information
Format Affinity Purified
Control
  • Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
Presentation Affinity Purified rabbit polyclonal IgG in PBS (without Mg2+ and Ca2+), pH 7.3 containing 0.5 mg/mL BSA (IgG, protease free) and 0.025% sodium azide and 50% glycerol.
Applications
Application Anti-phospho-Src (Tyr418) Antibody detects level of phospho-Src (Tyr418) & has been published & validated for use in IC, IH & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application Notes Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Src (Tyr418) in RIPA lysate of Src transfected CEF cells.
Biological Information
Immunogen Peptide corresponding to amino acid region encompassing the human, mouse, rat, chicken, and frog phospho-Src (Tyr418)
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Src phosphorylated on Tyrosine residue 418.
Isotype IgG
Species Reactivity
  • Chicken
  • Human
  • Mouse
Species Reactivity Note Predicted to cross-react with Rat and Frog (100% homologous) based on sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • SRC
  • ASV
  • c-src
  • p60-Src
  • SRC1
  • c-SRC
  • pp60c-src
Modifications
  • Phosphorylation
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight 60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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