Ms X MMP-2 (Gelatinase-A)

Species Reactivity	Key Applications	Host	Format	Antibody Type
Ch, H	ELISA, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Ch, H

ELISA, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB13435
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-MMP-2 Antibody, clone 7F3.2
Alternate Names	Gelatinase A 72 kDa Type IV Collagenase

Description

Catalogue Number

MAB13435

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-MMP-2 Antibody, clone 7F3.2

Alternate Names

Gelatinase A
72 kDa Type IV Collagenase

Product Information
Format	Purified
Presentation	Liquid at 1 mg/mL containing sodium azide as a preservative.

Product Information

Format

Purified

Presentation

Liquid at 1 mg/mL containing sodium azide as a preservative.

Applications
Application	Anti-MMP-2 Antibody, clone 7F3.2 is an antibody against MMP-2 for use in ELISA & WB.
Key Applications	ELISA Western Blotting
Application Notes	Ideal for Western Blotting and EIA applications. When used against the reduced protein, it identifies both a 72 kDa and a 68 kDa species (the pro-form and active form of the enzyme, respectively). Other applications have not been evaluated. Gelatinase-A is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen . The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media. Recommended antibody dilution for Western Blots is 1:1,000 when using colorimetric substrates such BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be required for non-human samples. Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-MMP-2 Antibody, clone 7F3.2 is an antibody against MMP-2 for use in ELISA & WB.

Key Applications

ELISA
Western Blotting

Application Notes

Ideal for Western Blotting and EIA applications. When used against the reduced protein, it identifies both a 72 kDa and a 68 kDa species (the pro-form and active form of the enzyme, respectively). Other applications have not been evaluated.

Gelatinase-A is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen . The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.

Recommended antibody dilution for Western Blots is 1:1,000 when using colorimetric substrates such BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be required for non-human samples.

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide based on the human sequence.
Clone	7F3.2
Host	Mouse
Specificity	Binds to Gelatinase-A. No cross reactivity with the other MMP family members (MMP-1, MMP-2B, MMP-3, and MMP-9).
Isotype	IgG1
Species Reactivity	Chicken Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_004530.2
Entrez Gene Summary	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome.
Gene Symbol	MMP2 TBE-1 CLG4A CLG4 MMP-II MONA MMP-2 EC 3.4.24.24
UniProt Number	P08253
UniProt Summary	FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond. COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit. SIZE: 660 amino acids; 73882 Da SUBUNIT: Ligand for integrin alpha-V/beta-3. TISSUE SPECIFICITY: Produced by normal skin fibroblasts. DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3). DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum. SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.

Biological Information

Immunogen

Synthetic peptide based on the human sequence.

Clone

7F3.2

Host

Mouse

Specificity

Binds to Gelatinase-A. No cross reactivity with the other MMP family members (MMP-1, MMP-2B, MMP-3, and MMP-9).

Isotype

IgG1

Species Reactivity

Chicken
Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_004530.2

Entrez Gene Summary

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome.

Gene Symbol

MMP2
TBE-1
CLG4A
CLG4
MMP-II
MONA
MMP-2
EC 3.4.24.24

UniProt Number

P08253

UniProt Summary

FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 660 amino acids; 73882 Da
SUBUNIT: Ligand for integrin alpha-V/beta-3.
TISSUE SPECIFICITY: Produced by normal skin fibroblasts.
DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).
DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	The undiluted antibody solution is stable for approximately 6 months when stored -20°C.

Storage and Shipping Information

Storage Conditions

The undiluted antibody solution is stable for approximately 6 months when stored -20°C.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Ms X MMP-2 (Gelatinase-A)

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