Anti-GluR4 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, M	IH(P), ICC, IHC, IP, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

R, M

IH(P), ICC, IHC, IP, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB1508
Replaces	06-308
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Glutamate Receptor 4 Antibody
Alternate Names	AMPA-selective glutamate receptor 4 Glutamate receptor ionotropic AMPA 4 glutamate receptor 4 glutamate receptor, ionotrophic AMPA 4
Background Information	Glutamate receptor 4 (UniProt: P48058; also known as GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D, Glutamate receptor ionotropic AMPA 4, GluA4) is encoded by the GRIA4 (also known as GLUR4) gene (Gene ID: 2893) in human. The postsynaptic actions of glutamate are mediated by a variety of receptors that are named according to their selective agonists. GluR-4 is a multi-pass membrane protein that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. It displays greater affinity for AMPA. GluR-4 is synthesized with a signal peptide (aa 1-20), which is cleaved off in the mature form. GluR-4 can exist as a homotetramer or heterotetramer of pore-forming glutamate receptor subunits, tetramers can also form by the dimerization of dimers. GluR-4 is generally palmitoylated and becomes de-palmitoylated upon glutamate stimulation. Palmitoylation on Cys 611 leads to its Golgi retention and reduced cell surface expression. However, palmitoylation at Cys 837 does not affect cell surface expression, but can regulate stimulation-dependent endocytosis. GluR-4 can undergo phosphorylation at Serine 862 by PKC gamma and this phosphorylation increases plasma membrane-associated GRI4 expression.

Description

Catalogue Number

AB1508

Replaces

06-308

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Glutamate Receptor 4 Antibody

Alternate Names

AMPA-selective glutamate receptor 4
Glutamate receptor ionotropic
AMPA 4
glutamate receptor 4
glutamate receptor, ionotrophic
AMPA 4

Background Information

Glutamate receptor 4 (UniProt: P48058; also known as GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D, Glutamate receptor ionotropic AMPA 4, GluA4) is encoded by the GRIA4 (also known as GLUR4) gene (Gene ID: 2893) in human. The postsynaptic actions of glutamate are mediated by a variety of receptors that are named according to their selective agonists. GluR-4 is a multi-pass membrane protein that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. It displays greater affinity for AMPA. GluR-4 is synthesized with a signal peptide (aa 1-20), which is cleaved off in the mature form. GluR-4 can exist as a homotetramer or heterotetramer of pore-forming glutamate receptor subunits, tetramers can also form by the dimerization of dimers. GluR-4 is generally palmitoylated and becomes de-palmitoylated upon glutamate stimulation. Palmitoylation on Cys 611 leads to its Golgi retention and reduced cell surface expression. However, palmitoylation at Cys 837 does not affect cell surface expression, but can regulate stimulation-dependent endocytosis. GluR-4 can undergo phosphorylation at Serine 862 by PKC gamma and this phosphorylation increases plasma membrane-associated GRI4 expression.

Product Information
Format	Affinity Purified
Control	Rat Brain
Presentation	Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Rat Brain

Presentation

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	This Anti-Glutamate Receptor 4 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 4.
Key Applications	Immunohistochemistry (Paraffin) Immunocytochemistry Immunohistochemistry Immunoprecipitation Western Blotting
Application Notes	Immunohistochemistry: Dilutions of 1:10 – 1:500 were tested on rat brain lysate. Can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Suggested concentrations start at 1-3 μg/mL. Western blot analysis can be done at final concentrations of 1-3 μg/mL. Optimal working dilutions must be determined by end user.

Applications

Application

This Anti-Glutamate Receptor 4 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 4.

Key Applications

Immunohistochemistry (Paraffin)
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blotting

Application Notes

Immunohistochemistry:
Dilutions of 1:10 – 1:500 were tested on rat brain lysate.
Can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Suggested concentrations start at 1-3 μg/mL.

Western blot analysis can be done at final concentrations of 1-3 μg/mL.

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Synthetic linear peptide.
Epitope	C-Terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Cat. # AB1508 recognizes C-Terminus of GluR4.
Species Reactivity	Rat Mouse
Species Reactivity Note	The immunogen sequence shares 100% conservation with chicken, mouse, rat, oppossum, human, chimp, and monkey.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000829.2 NM_001077243.1 NM_001077244.1
Entrez Gene Summary	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia.
Gene Symbol	GRIA4 GluR-D GLUR4C GLUR4 GluR4 GLURD GluR-4
Purification Method	Antigen Affinity Purified
UniProt Number	P48058
UniProt Summary	FUNCTION: SwissProt: P48058 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. SIZE: 902 amino acids; 100810 Da SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with EPB41L1 via its C-terminus (By similarity). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). SIMILARITY: SwissProt: P48058 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family. MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
Molecular Weight	Approx. 100 kDa

Biological Information

Immunogen

Synthetic linear peptide.

Epitope

C-Terminus

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Cat. # AB1508 recognizes C-Terminus of GluR4.

Species Reactivity

Rat
Mouse

Species Reactivity Note

The immunogen sequence shares 100% conservation with chicken, mouse, rat, oppossum, human, chimp, and monkey.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia.

Gene Symbol

GRIA4
GluR-D
GLUR4C
GLUR4
GluR4
GLURD
GluR-4

Purification Method

Antigen Affinity Purified

UniProt Number

P48058

UniProt Summary

FUNCTION: SwissProt: P48058 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
SIZE: 902 amino acids; 100810 Da
SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with EPB41L1 via its C-terminus (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
SIMILARITY: SwissProt: P48058 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

Molecular Weight

Approx. 100 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in mouse and rat brain membrane. Western Blotting Analysis: A 1:500 dilution of this antibody detected Glutamate Receptor 4 in mouse and rat brain membrane.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in mouse and rat brain membrane.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Glutamate Receptor 4 in mouse and rat brain membrane.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-GluR4 (rabbit polyclonal)

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