Rb X AIF

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IH(P), WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

IH(P), WB

Purified

Polyclonal Antibody

Description
Catalogue Number	AB16501
Replaces	04-430
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-AIF Antibody, internal domain
Alternate Names	Apoptosis Inducing Factor
Background Information	Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami et al., 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al., 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al., 1999).

Description

Catalogue Number

AB16501

Replaces

04-430

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-AIF Antibody, internal domain

Alternate Names

Apoptosis Inducing Factor

Background Information

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami et al., 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al., 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al., 1999).

Product Information
Format	Purified
Control	Widely expressed
Presentation	Purified IgG in PBS containing 0.02% sodium azide.

Product Information

Format

Purified

Control

Widely expressed

Presentation

Purified IgG in PBS containing 0.02% sodium azide.

Applications
Application	Anti-AIF Antibody, internal domain is an antibody against AIF for use in IH(P) & WB.
Key Applications	Immunohistochemistry (Paraffin) Western Blotting
Application Notes	Western blot: 0.25 to 1 μg/mL. K562 cell lysate can be used as a positive control and a 67 kDa band should be detected. Immunohistochemistry on formalin fixed, paraffin sections: 5-20 μg/ml, with antigen retrieval; see website protocol for details and photographs. Optimal working dilutions must be determined by end user.

Applications

Application

Anti-AIF Antibody, internal domain is an antibody against AIF for use in IH(P) & WB.

Key Applications

Immunohistochemistry (Paraffin)
Western Blotting

Application Notes

Western blot: 0.25 to 1 μg/mL.

K562 cell lysate can be used as a positive control and a 67 kDa band should be detected.

Immunohistochemistry on formalin fixed, paraffin sections: 5-20 μg/ml, with antigen retrieval; see website protocol for details and photographs.

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Rabbit anti-AIF (Internal region) polyclonal antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF (Susin et al. 1999). This sequence is identical to those of mouse and rat AIF (Susin et al. 1999).
Epitope	internal domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al. 1999).
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004208.2 NM_145812.1 NM_145813.1
Entrez Gene Summary	This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.
Gene Symbol	AIFM1 MGC111425 PDCD8 AIF EC 1.-.-.-
Purification Method	Ammonium sulfate precipitation and DEAE-cellulose chromatography
UniProt Number	O95831
UniProt Summary	FUNCTION: SwissProt: O95831 # Probable oxidoreductase that acts as a caspase- independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro). COFACTOR: FAD. SIZE: 613 amino acids; 66901 Da SUBUNIT: Binds to DNA in a sequence-independent manner. SUBCELLULAR LOCATION: Mitochondrion intermembrane space. Nucleus. Note=Translocated to the nucleus upon induction of apoptosis. SIMILARITY: SwissProt: O95831 ## Belongs to the FAD-dependent oxidoreductase family.
Molecular Weight	67 kDa

Biological Information

Immunogen

Rabbit anti-AIF (Internal region) polyclonal antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF (Susin et al. 1999). This sequence is identical to those of mouse and rat AIF (Susin et al. 1999).

Epitope

internal domain

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al. 1999).

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.

Gene Symbol

AIFM1
MGC111425
PDCD8
AIF
EC 1.-.-.-

Purification Method

Ammonium sulfate precipitation and DEAE-cellulose chromatography

UniProt Number

O95831

UniProt Summary

FUNCTION: SwissProt: O95831 # Probable oxidoreductase that acts as a caspase- independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro).
COFACTOR: FAD.
SIZE: 613 amino acids; 66901 Da
SUBUNIT: Binds to DNA in a sequence-independent manner.
SUBCELLULAR LOCATION: Mitochondrion intermembrane space. Nucleus. Note=Translocated to the nucleus upon induction of apoptosis.
SIMILARITY: SwissProt: O95831 ## Belongs to the FAD-dependent oxidoreductase family.

Molecular Weight

67 kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Storage and Shipping Information

Storage Conditions

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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