Anti-GSK3b (rabbit polyclonal)

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Anti-GSK3b (rabbit polyclonal)

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货号：07-1413

品牌：Millipore 密理博

配送至：

北京朝阳

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数量：

EA (预计3-5工作日到货)

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Replacement Information
Replacement Information	07-1413 is a recommended replacement for AB8687

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, H, M, B	WB, IP, IH(P)	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	07-1413
Replaces	AB8687
Description	Anti-GSK-3b Antibody, a.a. 335-349
Background Information	GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.

References

Product Information
Format	Affinity Purified
Control	C2C12 Cell Lysate, Hela cells, rat or mouse brain
Presentation	Purified polyclonal IgG antibody is supplied in PBS, pH 7.2, 50% glycerol and 0.09% sodium azide.

Applications
Application	This AntiI-GSK-3b Antibody, a.a. 335-349 is validated for use in WB, IP, IH(P) for the detection of AntiI-GSK-3b.
Key Applications	Western Blotting Immunoprecipitation Immunohistochemistry (Paraffin)
Application Notes	Immunoprecipitation: 12.5 µg/mL dilution of a previous lot was ued in immunoprecipitation on rat brain tissue; immunoprecipitations with tissue extracts other than brain may require higher antibody concentrations. Optimal working dilutions must be determined by the end user. Immunohistochemistry(paraffin): Representative testing from a previous lot. Optimal Staining of GSK3β Polyclonal Antibody: HeLa Cells

Biological Information
Immunogen	Rat GSK3 beta synthetic peptide (aa. 335-349), conjugated to KLH. This sequence is 100% identical to human GSK3 beta.
Epitope	a.a. 335-349
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody detects a strong 47 kDa protein, corresponding to the expected molecular mass of GSK3 beta.
Isotype	IgG
Species Reactivity	Rat Human Mouse Bovine
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_002093
Entrez Gene Summary	Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and nactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed1333807]).[supplied by OMIM].
Gene Symbol	GSK3B EC 2.7.11.26
Purification Method	Protein A affinity purified
UniProt Number	P49841
UniProt Summary	FUNCTION: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. CATALYTIC ACTIVITY: ATP + [tau protein] = ADP + [tau protein] phosphate. ENZYME REGULATION: Inhibited when phosphorylated by AKT1. SUBUNIT: Monomer (By similarity). Interacts with CABYR, MUC1, NIN and PRUNE. ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
Molecular Weight	This antibody detects a strong 47 kDa protein.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on C2C12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected GSK3 BETA on 10 μg of C2C12 lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable at –20 ºC for up to one year from date of receipt. Product may be stored at for 2-4 weeks at 2º-8ºC. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable at –20 ºC for up to one year from date of receipt. Product may be stored at for 2-4 weeks at 2º-8ºC.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.