Chk X GFP

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首页克隆与表达Millipore 密理博>

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货号：AB16901

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
Vrt	ICC, IP, WB	Ch	Purified	Polyclonal Antibody

Description
Catalogue Number	AB16901
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Green Fluorescent Protein Antibody
Alternate Names	GFP eGFP
Background Information	The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

References

Product Information
Format	Purified
Control	GFP fusion protein expressed in cells
Presentation	Purified chicken immunoglobulin in TBS, pH 7.5 with 0.02% sodium azide.

Applications
Application	This Anti-Green Fluorescent Protein Antibody is validated for use in IC, IP, WB for the detection of Green Fluorescent Protein.
Key Applications	Immunocytochemistry Immunoprecipitation Western Blotting
Application Notes	Immunoblotting: AB16901 detects a band of the proper molecular weight (30kDa) in lysates from E.coli expressing GFP but not in E.coli that do not express or in lysates prepared from E.coli containing the GFP-expressing plasmid but not induced, detect GFP in lysates from COS or primary neurons or fibroblast cells transfected with plasmids directing expression GFP or a tau-GFP fusion protein. Did not detect any signal when 50μg of protein from a rat brain lysate (negative control) was fractionated on the SDS-PAGE. Titers were 10-20,000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB. Immunocytochemistry: GFP can be detected in cells that are transfected with a plasmid directing the expression of either GFP or a GFP-Fusion protein. Cells that do not express GFP exhibit no detectable staining. AB binding was determined using HRP conjugated-anti chicken secondary Abs and visualized using DAB. The anti-GFP Abs were used at 1-2,500-5,000 fold dilution. Cells were fixed using 4% formaldehyde in PBS (pH7.4). For immunocytochemistry, the cultures were fixed in 4% formaldehyde in PBS pH7.4, for 20 minutes at room temperature. The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the aminal in which the secondary antibody was raised) in PBS for 20 minutes at room temperature. Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4°C, overnight. Antibody binding is detected using an avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine / 0.02% hydrogen peroxide solution on ice for 8-10 minutes. AB16901 has been used to detect GFP in primary neurons and zebrafish fibroblasts. Immunoprecipitation: 5μg antibody per no more than 500μl of cell lysate. It is important to use a rabbit anti-chicken secondary for capture or anti-chicken beads as Chicken IgG will not react with protein A or protein G. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	A recombinant green fluorescent protein (GFP) containing a 6-his tag, was highly purified by affinity chromatography on Nickel column followed by chromatography on a P-100 gel filtration column. The resulting product was present as only a singl
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Chicken
Specificity	AB16901 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources.
Species Reactivity	Vertebrates
Antibody Type	Polyclonal Antibody
Purification Method	Ammonium Sulfate Precipitation
UniProt Number	P42212
Molecular Weight	MW for GFP is 27 kDa. Dependent upon the molecular weight of the fusion protein being detected.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8°C. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Storage and Shipping Information

Storage Conditions

The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8°C.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.