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Anti phospho-Src family (Tyr416), clone 2N8

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货号：05-857

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Vrt, M WB Rb Culture Supernatant Monoclonal Antibody
Description
Catalogue Number 04-857
Replaces 05-857
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
Alternate Names
  • Proto-oncogene tyrosine-protein kinase Src
  • Proto-oncogene c-Src
  • pp60c-src
  • p60-Src
Background Information Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
References
Product Information
Format Culture Supernatant
Control
  • RIPA cell lysate
Presentation Cultured supernantant in 0.05% sodium azide
Applications
Application Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB.
Key Applications
  • Western Blotting
Application Notes Western Blot Analysis:
A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A).

Immunoprecipitation:
A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B).
Biological Information
Immunogen KLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src
Clone 2N8
Host Rabbit
Specificity phospho-c-Src (Tyr416)
Isotype IgG
Species Reactivity
  • Vertebrates
  • Mouse
Species Reactivity Note Mouse. Broad species cross-reactivity expected based on sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • pp60c-src
  • ASV
  • c-SRC
  • SRC1
  • p60-Src
  • c-src
  • SRC
Modifications
  • Phosphorylation
UniProt Number
UniProt Summary SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight 60kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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