Anti-tPA (Tissue Plasminogen Activator), clone GMA-043

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ELISA, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

ELISA, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-883
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-tPA (Tissue Plasminogen Activator) Antibody, clone GMA-043

Description

Catalogue Number

05-883

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-tPA (Tissue Plasminogen Activator) Antibody, clone GMA-043

Product Information
Format	Purified
Presentation	100 µg of protein G purified mouse IgG1 lyophilized from 10 mM NaH₂PO₄, 0.15 M NaCl, 1.0% mannitol, pH 7.4.

Product Information

Format

Purified

Presentation

100 µg of protein G purified mouse IgG1 lyophilized from 10 mM NaH₂PO₄, 0.15 M NaCl, 1.0% mannitol, pH 7.4.

Applications
Application	Detect tPA (Tissue Plasminogen Activator) using this Anti-tPA (Tissue Plasminogen Activator) Antibody, clone GMA-043 validated for use in ELISA & WB.
Key Applications	ELISA Western Blotting

Applications

Application

Detect tPA (Tissue Plasminogen Activator) using this Anti-tPA (Tissue Plasminogen Activator) Antibody, clone GMA-043 validated for use in ELISA & WB.

Key Applications

ELISA
Western Blotting

Biological Information
Immunogen	Purified human tissue plasminogen activator (tPA)
Clone	clone GMA-043
Host	Mouse
Specificity	tPA
Isotype	IgG1
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000930
Entrez Gene Summary	This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Gene Symbol	PLAT Reteplase reteplase T-PA alteplase DKFZp686I03148 Alteplase tPA TPA
Purification Method	Protein G Purified
UniProt Number	P00750
UniProt Summary	FUNCTION: SwissProt: P00750 # Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. SIZE: 562 amino acids; 62917 Da SUBUNIT: Heterodimer of chain A and chain B held by a disulfide bond. Binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-fold and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binds to annexin A2, cytokeratin-8, fibronectin and laminin. Binds to mannose receptor and the low- density lipoprotein receptor-related protein (LRP1); these proteins are involved in TPA clearance. Yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold. Binds LRP1B; binding is followed by internalization and degradation. SUBCELLULAR LOCATION: Secreted, extracellular space. TISSUE SPECIFICITY: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. DOMAIN: SwissProt: P00750 Both FN1 and one of the kringle domains are required for binding to fibrin. & Both FN1 and EGF-like domains are important for binding to LRP1. & The FN1 domain mediates binding to annexin A2. & The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. PTM: The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa. & Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two- chain type II glycoform in the presence of fibrin. & N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor. & Characterization of O-linked glycan was studied in Bowes melanoma cell line.DISEASE:SwissProt: P00750 # Increased activity of TPA causes hyperfibrinolysis, with excessive bleeding as a consequence. & Defective release of TPA causes hypofibrinolysis, leading to thrombosis or embolism. SIMILARITY: Belongs to the peptidase S1 family. & Contains 1 EGF-like domain. & Contains 1 fibronectin type-I domain. & Contains 2 kringle domains. & Contains 1 peptidase S1 domain.
Molecular Weight	Mr 63kDa

Biological Information

Immunogen

Purified human tissue plasminogen activator (tPA)

Clone

clone GMA-043

Host

Mouse

Specificity

tPA

Isotype

IgG1

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000930

Entrez Gene Summary

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Gene Symbol

PLAT
Reteplase
reteplase
T-PA
alteplase
DKFZp686I03148
Alteplase
tPA
TPA

Purification Method

Protein G Purified

UniProt Number

P00750

UniProt Summary

FUNCTION: SwissProt: P00750 # Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
SIZE: 562 amino acids; 62917 Da
SUBUNIT: Heterodimer of chain A and chain B held by a disulfide bond. Binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-fold and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binds to annexin A2, cytokeratin-8, fibronectin and laminin. Binds to mannose receptor and the low- density lipoprotein receptor-related protein (LRP1); these proteins are involved in TPA clearance. Yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold. Binds LRP1B; binding is followed by internalization and degradation.
SUBCELLULAR LOCATION: Secreted, extracellular space.
TISSUE SPECIFICITY: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
DOMAIN: SwissProt: P00750 Both FN1 and one of the kringle domains are required for binding to fibrin. & Both FN1 and EGF-like domains are important for binding to LRP1. & The FN1 domain mediates binding to annexin A2. & The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
PTM: The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa. & Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two- chain type II glycoform in the presence of fibrin. & N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor. & Characterization of O-linked glycan was studied in Bowes melanoma cell line.DISEASE:SwissProt: P00750 # Increased activity of TPA causes hyperfibrinolysis, with excessive bleeding as a consequence. & Defective release of TPA causes hypofibrinolysis, leading to thrombosis or embolism.
SIMILARITY: Belongs to the peptidase S1 family. & Contains 1 EGF-like domain. & Contains 1 fibronectin type-I domain. & Contains 2 kringle domains. & Contains 1 peptidase S1 domain.

Molecular Weight

Mr 63kDa

Product Usage Statements
Quality Assurance	routinely evaluated by immunoblot on human tPA
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

routinely evaluated by immunoblot on human tPA

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	stable 2 years at -20°C from date of shipment

Storage and Shipping Information

Storage Conditions

stable 2 years at -20°C from date of shipment

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-tPA (Tissue Plasminogen Activator), clone GMA-043

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