Rb X CXCR4 Extracellular Loop

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

Purified

Polyclonal Antibody

Description
Catalogue Number	AB1847
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop
Alternate Names	CD184 CXCR4

Description

Catalogue Number

AB1847

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop

Alternate Names

CD184
CXCR4

Product Information
Format	Purified
Presentation	Purified IgG in PBS containing 0.02% sodium azide.

Product Information

Format

Purified

Presentation

Purified IgG in PBS containing 0.02% sodium azide.

Applications
Application	Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop detects level of C-X-C Chemokine Receptor 4 & has been published & validated for use in WB.
Key Applications	Western Blotting
Application Notes	Western blot: 1:500 - 1000 Optimal working dilutions must be determined by end user.

Applications

Application

Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop detects level of C-X-C Chemokine Receptor 4 & has been published & validated for use in WB.

Key Applications

Western Blotting

Application Notes

Western blot: 1:500 - 1000

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Extracellular loop peptide of human CXCR4 (Berson et al. 1996; Leotscher et al. 1994).
Epitope	extracellular loop
Host	Rabbit
Specificity	Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (Nimitrov 1997). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principle coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (Feng et al. 1996; Berson et al. 1996). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (Doranz et al. 1996; Endres et al. 1996). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (Bleul et al. 1996; Oberlin et al. 1996). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (Lapham et al. 1996). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (Feng et al. 1996). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (Feng et al. 1996; Endres et al. 1996; Brelot et al. 1997). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (Brelot et al. 1997; Lu et al. 1997).
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001008540.1 NM_003467.2
Entrez Gene Summary	This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene Symbol	CXCR4 WHIM NPY3R NPYY3R FB22 LAP3 Fusin CXCR-4 LCR1 NPYR HM89 fusin HSY3RR CXC-R4 NPYY3 NPYRL D2S201E CD184 LESTR
UniProt Number	P61073
UniProt Summary	FUNCTION: SwissProt: P61073 # Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. SIZE: 352 amino acids; 39746 Da SUBUNIT: Interacts with HIV-1 surface protein gp120 and Tat. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. DOMAIN: SwissProt: P61073 The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity. PTM: Sulfated. DISEASE: SwissProt: P61073 # Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also called warts, hypogammaglobulinemia, infections, and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis. SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

Biological Information

Immunogen

Extracellular loop peptide of human CXCR4 (Berson et al. 1996; Leotscher et al. 1994).

Epitope

extracellular loop

Host

Rabbit

Specificity

Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (Nimitrov 1997). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principle coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (Feng et al. 1996; Berson et al. 1996). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (Doranz et al. 1996; Endres et al. 1996). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (Bleul et al. 1996; Oberlin et al. 1996). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (Lapham et al. 1996). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (Feng et al. 1996). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (Feng et al. 1996; Endres et al. 1996; Brelot et al. 1997). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (Brelot et al. 1997; Lu et al. 1997).

Species Reactivity

Human
Mouse

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene Symbol

CXCR4
WHIM
NPY3R
NPYY3R
FB22
LAP3
Fusin
CXCR-4
LCR1
NPYR
HM89
fusin
HSY3RR
CXC-R4
NPYY3
NPYRL
D2S201E
CD184
LESTR

UniProt Number

P61073

UniProt Summary

FUNCTION: SwissProt: P61073 # Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
SIZE: 352 amino acids; 39746 Da
SUBUNIT: Interacts with HIV-1 surface protein gp120 and Tat.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
DOMAIN: SwissProt: P61073 The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
PTM: Sulfated.
DISEASE: SwissProt: P61073 # Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also called warts, hypogammaglobulinemia, infections, and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Can be refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Storage and Shipping Information

Storage Conditions

Can be refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Rb X CXCR4 Extracellular Loop

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