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PAK1-PBD, agarose free

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货号：14-864

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 14-864
Brand Family Upstate
Trade Name
  • Upstate
Description PAK-1 PBD Protein (agarose free), 300 µg
Alternate Names
  • p21-activated kinase 1
  • p21/Cdc42/Rac1-activated kinase 1
References
Product Information
Presentation 300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.
Applications
Application For use in Affinity Binding Assays, ELISA & G-Protein Assays.
Key Applications
  • Affinity Binding Assay
  • ELISA
  • G-Protein Assay
Biological Information
Concentration 2 mg/mL
Purity Purity >50% by SDS-PAGE and Coomassie blue staining.
Source GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.
Specific Activity Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.
Entrez Gene Number
Entrez Gene Summary PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures have not yet been determined.
Gene Symbol
  • PAK1
  • p65-PAK
  • MGC130001
  • Alpha-PAK
  • MGC130000
  • PAK-1
  • PAKalpha
Purification Method GSH-agarose chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q13153 # The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
COFACTOR: Magnesium.
SIZE: 545 amino acids; 60647 Da
SUBUNIT: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1.
SUBCELLULAR LOCATION: Cytoplasm. Cell junction, focal adhesion. Note=Recruited to focal adhesions upon activation.
PTM: Autophosphorylated when activated by CDC42/p21 and RAC1.
SIMILARITY: SwissProt: Q13153 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CRIB domain. & Contains 1 protein kinase domain.
Molecular Weight 21 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by G-protein activity assay
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Packaging Information
Material Size 300 µg
Transport Information
Supplemental Information
Specifications

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